Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates

Ataollahi, F. and Pramanik, S. and Moradi, A. and Dalilottojari, A. and Pingguan-Murphy, Belinda and Wan Abas, Wan Abu Bakar and Abu Osman, Noor Azuan (2015) Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates. Journal of Biomedical Materials Research Part A, 103 (7). pp. 2203-2213. ISSN 1549-3296, DOI https://doi.org/10.1002/jbm.a.35186.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/24733741


Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2O3) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt Al2O3 at a curing temperature of 50 degrees C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1-dioctadecyl-1,3,3,3,3-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt Al2O3) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2203-2213, 2015.

Item Type: Article
Funders: Ministry of Higher Education (MOHE) of Malaysia UM.C/HIR/MOHE/ENG/14 D000014-16001 , University of Malaya Faculty of Engineering UM-TNC2/IPPP/PPGP/638/PPP/PG0872013B
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Uncontrolled Keywords: Stiffness, proliferation, bovine aortic endothelial cells, extra, cellular matrix, vessels, NITRIC-OXIDE SYNTHASE, X-RAY-DIFFRACTION, POROUS SILICON, WATER-VAPOR, ALUMINA, SURFACES, ALPHA,
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 17 Mar 2016 01:28
Last Modified: 25 Oct 2018 08:44
URI: http://eprints.um.edu.my/id/eprint/15718

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