Ataollahi, F. and Pramanik, S. and Moradi, A. and Dalilottojari, A. and Pingguan-Murphy, Belinda and Wan Abas, Wan Abu Bakar and Abu Osman, Noor Azuan (2015) Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates. Journal of Biomedical Materials Research Part A, 103 (7). pp. 2203-2213. ISSN 1549-3296, DOI https://doi.org/10.1002/jbm.a.35186.
Full text not available from this repository.Abstract
Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2O3) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt Al2O3 at a curing temperature of 50 degrees C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1-dioctadecyl-1,3,3,3,3-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt Al2O3) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2203-2213, 2015.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education (MOHE) of Malaysia UM.C/HIR/MOHE/ENG/14 D000014-16001 , University of Malaya Faculty of Engineering UM-TNC2/IPPP/PPGP/638/PPP/PG0872013B |
| Additional Information: | ISI Document Delivery No.: CJ1LZ Times Cited: 0 Cited Reference Count: 43 Cited References: Vasquez-A MA, 2007, REV MEX FIS, V53, P431 Ataollahi F, 2014, CYTOTHERAPY, V16, P1145, DOI 10.1016/j.jcyt.2014.01.010 AWOLESI MA, 1995, J CLIN INVEST, V96, P1449, DOI 10.1172/JCI118181 Boumaza A, 2009, J SOLID STATE CHEM, V182, P1171, DOI 10.1016/j.jssc.2009.02.006 Califano JP, 2008, CELL MOL BIOENG, V1, P122, DOI 10.1007/s12195-008-0022-x Cines DB, 1998, BLOOD, V91, P3527 Engler AJ, 2006, CELL, V126, P677, DOI 10.1016/j.cell.2006.06.044 Feret FR, 2000, SPECTROCHIM ACTA B, V55, P1051, DOI 10.1016/S0584-8547(00)00225-1 Gang EJ, 2004, BIOCHEM BIOPH RES CO, V321, P102, DOI 10.1016/j.bbrc.2004.06.111 Huh D, 2013, NAT PROTOC, V8, P2135, DOI 10.1038/nprot.2013.137 Jantova S., 2011, ACTA CHIMICA SLOVACA, V4, P15 Johansson F, 2009, ACTA BIOMATER, V5, P2230, DOI 10.1016/j.actbio.2009.02.010 Kamgoue A, 2006, J BIOMECH ENG, V129, P523 Katamreddy R, 2006, J ELECTROCHEM SOC, V153, pC701, DOI 10.1149/1.2239258 Khumrak S., 2012, BANGK MED J, V4, P93 Kloprogge JT, 2004, J RAMAN SPECTROSC, V35, P967, DOI 10.1002/jrs.1244 Lee JN, 2004, LANGMUIR, V20, P11684, DOI 10.1021/la048562+ Leukers B, 2005, MATERIALWISS WERKST, V36, P781, DOI 10.1002/mawe.200500968 Lin SL, 1996, J NON-CRYST SOLIDS, V202, P61, DOI 10.1016/0022-3093(96)00138-X Mason B, 2012, ENG BIOMATERIALS REG, P19 Mata A, 2005, BIOMED MICRODEVICES, V7, P281, DOI 10.1007/s10544-005-6070-2 Mehta J, 2013, ADV BIOCH HLTH DIS Ochoa M, 2012, J SOL-GEL SCI TECHN, V61, P151, DOI 10.1007/s10971-011-2604-7 Paszek MJ, 2005, CANCER CELL, V8, P241, DOI 10.1016/j.ccr.2005.08.010 Pelham RJ, 1997, P NATL ACAD SCI USA, V94, P13661, DOI 10.1073/pnas.94.25.13661 Pramanik S, 2012, SCI TECHNOL ADV MAT, V13, DOI 10.1088/1468-6996/13/4/043002 Ramaswamy K, 2010, AM J PHYSIOL-CELL PH, V300, P146 Shivakumar E, 2005, MACROMOL RES, V13, P81 Shum D, 2008, J ENZYM INHIB MED CH, V23, P931, DOI 10.1080/14756360701810082 Sluimer JC, 2009, J PATHOL, V218, P7, DOI 10.1002/path.2518 Stroka KM, 2011, BLOOD, V118, P1632, DOI 10.1182/blood-2010-11-321125 Su XH, 2011, J MATER SCI TECHNOL, V27, P1011 Sunyer R, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0046107 Takeda H, 2006, LIFE SCI, V79, P233, DOI 10.1016/j.lfs.2005.12.051 Tan PS, 2007, MAT SCI ENG C-BIO S, V27, P304, DOI 10.1016/j.msec.2006.03.010 Wang H, 2007, CARDIOVASC RES, V73, P497, DOI 10.1016/j.cardiores.2006.10.007 Weijia Z, 2013, SCI REP, V3, P2332 Wells RG, 2008, HEPATOLOGY, V47, P1394, DOI 10.1002/hep.22193 Yang CY, 2010, EUR CELLS MATER, V20, P415 Yeh YT, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0046889 Yeung T, 2005, CELL MOTIL CYTOSKEL, V60, P24, DOI 10.1002/cm.20041 Zeng DH, 2010, BIOMECH MODEL MECHAN, V9, P19, DOI 10.1007/s10237-009-0156-3 Ziegler T, 1998, ARTERIOSCL THROM VAS, V18, P686 Ataollahi, Forough Pramanik, Sumit Moradi, Ali Dalilottojari, Adel Pingguan-Murphy, Belinda Abas, Wan Abu Bakar Wan Abu Osman, Noor Azuan Engineering, Faculty /I-7935-2015 Engineering, Faculty /0000-0002-4848-7052 Ministry of Higher Education (MOHE) of Malaysia UM.C/HIR/MOHE/ENG/14 D000014-16001; University of Malaya Faculty of Engineering UM-TNC2/IPPP/PPGP/638/PPP/PG0872013B Contract grant sponsor: Ministry of Higher Education (MOHE) of Malaysia; contract grant number: UM.C/HIR/MOHE/ENG/14 D000014-16001Contract grant sponsor: University of Malaya Faculty of Engineering; contract grant number: UM-TNC2/IPPP/PPGP/638/PPP/PG0872013B 0 WILEY-BLACKWELL HOBOKEN J BIOMED MATER RES A |
| Uncontrolled Keywords: | Stiffness, proliferation, bovine aortic endothelial cells, extra, cellular matrix, vessels, NITRIC-OXIDE SYNTHASE, X-RAY-DIFFRACTION, POROUS SILICON, WATER-VAPOR, ALUMINA, SURFACES, ALPHA, |
| Subjects: | T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) |
| Divisions: | Faculty of Engineering |
| Depositing User: | Mr Jenal S |
| Date Deposited: | 17 Mar 2016 01:28 |
| Last Modified: | 25 Oct 2018 08:44 |
| URI: | http://eprints.um.edu.my/id/eprint/15718 |
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