Lab-on-a-disk as a potential microfluidic platform for dengue NS1-ELISA

Yusoff, N.A. and Soin, N. and Ibrahim, F. (2009) Lab-on-a-disk as a potential microfluidic platform for dengue NS1-ELISA. In: 2009 IEEE Symposium on Industrial Electronics and Applications, ISIEA 2009, 2009, Kuala Lumpur.

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Detection of non-structural protein 1 (NS1) of dengue virus can gives early diagnosis of dengue and shows high sensitivity. Current technique uses for the dengue NS1 detection is enzyme-linked-immusorbent assay (ELISA) on 96 microwell plate. However, the assay requires long incubation time about 90 minutes (for antigen-antibody interaction) and total assay time takes almost 2 hours and 30 minutes to complete. Therefore, a lab-on-a-disk with applied centrifugal force is proposed as a potential microfluidic platform to reduce the assay time by effectively mix and separate liquid in the ELISA assay. The advantages of the technique are having large specific volume, short diffusion length, minimum reagents consumption and simplify procedures. The lab-on-a-disk will exploit centrifugal and capillary forces to act as a passive valve to control the flow sequence of different solutions involved. Each steps of the ELISA process is carried out automatically by controlling the rotation speed of the disk. This paper will describe the lab-on-a-disk platform on its microfluidic principles, fabrication process, detection systems, biosensor applications, and the proposed model for dengue NS1-ELISA assay. © 2009 IEEE.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Conference code: 79286 Cited By (since 1996):4 Export Date: 29 January 2014 Source: Scopus Art. No.: 5356330 doi: 10.1109/ISIEA.2009.5356330 Language of Original Document: English Correspondence Address: Yusoff, N. A.; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia; email: References: Xu, H., Di, B., Pan, Y., Qiu, L., Wang, Y., Hao, W., Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections (2006) J. Clin. Microbiol., 44, pp. 2872-2878. , May; Kumarasamy, V., Wahab, A.H.A., Chua, S.K., Hassan, Z., Chem, Y.K., Mohamad, M., Chua., K.B., Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection (2006) J. Virol. Methods, 140, pp. 75-79. , November; Rossier, J.S., Girault, H.H., Enzyme linked immunosorbent assay on a microchip with electrochemical detection (2001) Lab on a Chip, 1, pp. 153-157; Lai, S., Wang, S., Luo, J., Lee, L.J., Yang, S.-T., Madou, M.J., Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay (2004) Analytical Chemistry, 76 (7), pp. 1832-1837. , DOI 10.1021/ac0348322; Madou, M.J., Lee, L.J., Daunert, S., Lai, S., Shih, C.-H., Design and fabrication of CD-like microfluidic platforms for diagnostic: Mircofluidic functions (2001) Biomedical Microdevices, 3 (3), pp. 245-254. , DOI 10.1023/A:1011419515576; Riegger, L., Grumann, M., Nann, T., Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms (2005) Sensors and Actuators, 126 (2006), pp. 455-462. , December; Lee, L.J., Madou, M.J., Koelling, K.W., Daunert, S., Lai, S., Koh, C.G., Juang, Y.-J., Yu, L., Design and fabrication of CD-like microfluidic platforms for diagnostics: Polymer-based microfabrication (2001) Biomedical Microdevices, 3 (4), pp. 339-351. , DOI 10.1023/A:1012469017354; Roberts, M.A., Rossier, J.S., Bercier, P., Girault, H., UV laser machined polymer substrates for the development of microdiagnostic systems (1997) Anal. Chem., 69, pp. 2035-2042; Madou, M.J., (1997) Fundamentals of Microfabrication, , CRC Press, Boca Raton; Elizabeth, A., Moschou, Nicholson, A.D., Jia, G., Zoval, J.V., Madou, M.J., Integration of microcolumns and microfluidic fractionators on multitasking centrifugal microfluidic platform for the analysis of biomolecules (2006) Anal. Bioanal. Chem., 385, pp. 596-605. , May; Morais, S., Carracosa, J., Mira, D., Puchades, R., Maquieira, A., Microimmunoanalysis on standard compact discs to determine low abundant compounds (2007) Anal. Chem., 79 (20), pp. 7628-7635; Nagai, H., Narita, Y., Ohtaki, M., Saito, K., Wakida, M., A single bead analysis on disk shaped microfluidic (2007) Japan Society for Analytical Chemistry, 23, pp. 975-979. , Aug; Steigert, J., Grumann, M., Brenner, T., Riegger, L., Harter, J., Zengerle, R., Ducree, J., Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform (2006) Lab on a Chip, 6 (8), pp. 1040-1044. , May; Steinert, C.P., Mueller-Dieckmann, J., Weiss, M., Roessle, M., Zengerle, R., Koltay, P., Miniaturized and highly parallel protein crystallization on a microfluidic disc (2007) Proceedings of 19th International Conference on Micro Electro Mechanical Systems (MEMS 2007), pp. 561-564. , Kobe, Japan; Li, C., Dong, X., Qin, J., Lin, B., Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device (2009) Analytica Chimica Acta, 640, pp. 93-99. , March Sponsors: IEEE Malaysia Section; IEEE Malaysia PEL/IE/IA Joint Chapter; IEEE Malaysia Engineering in Medicine and Biology Chapter; IEEE Malaysia Instrumentation and Measurement Chapter
Uncontrolled Keywords: Centrifugal and capillary force, Dengue NS1-ELISA, Lab-on-a-disk, 96-Microwell, Antigen-antibody interaction, Biosensor applications, Capillary force, Centrifugal Forces, Current techniques, Dengue virus, Detection system, Early diagnosis, ELISA assay, Fabrication process, High sensitivity, Incubation time, Microfluidic platforms, Passive valves, Rotation speed, Short diffusion lengths, Specific volume, Structural proteins, Biosensors, Centrifugation, Computer viruses, Industrial electronics, Laboratories, Microfluidics, Viruses, Disks (structural components)
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 26 Mar 2014 02:18
Last Modified: 01 Nov 2017 04:06

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