Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production

Husin, Maryam and Rahim, Nurnadiah and Ahmad, Mohd Radzi and Romli, Ahmad Zafir and Ilham, Zul (2019) Hydrolysis of microcrystalline cellulose isolated from waste seeds of Leucaena leucocephala for glucose production. Malaysian Journal of Fundamental and Applied Sciences, 15 (2). pp. 200-205. ISSN 2289-5981, DOI https://doi.org/10.11113/mjfas.v15n2.1165.

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Official URL: https://doi.org/10.11113/mjfas.v15n2.1165


The waste seeds of Leucaena leucocephala (LLS) used in this study were unused residues obtained after oil and polysaccharides extraction. The microcrystalline cellulose (MCC) was isolated from LLS by acid treatment. MCC produced was, then, further converted to glucose by using sulphuric acid at 121 °C by varying the acid concentration and reaction time. The sugar composition was analyzed by using the phenol-sulfuric acid method and pre-column derivatization HPLC technique. The yield of glucose ranging from 70–85% could be obtained from MCC hydrolyzates, depending on the hydrolysis factors, which corresponding to around 57-75% of the percentage conversion of MCC to glucose.Cellulose isolated from LLS was, therefore, potentially suitable to be utilized in liquid biofuels and other value-added chemicals such as bioethanol, 5-hydroxymethylfurfural(HMF), and levulinic acid.

Item Type: Article
Funders: University of Malaya and Sustainability Science Research Cluster: research funding RP004B-13BIO and PG101-2015A
Uncontrolled Keywords: Leucaena leucocephala seed; waste residue; micro cellulose; acid hydrolysis; glucose
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
T Technology > T Technology (General)
T Technology > TD Environmental technology. Sanitary engineering
T Technology > TP Chemical technology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Dr. Zul Ilham
Date Deposited: 09 Apr 2020 04:33
Last Modified: 09 Apr 2020 04:33
URI: http://eprints.um.edu.my/id/eprint/24162

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