Short communication: energetics of glucoamylase-catalyzed hydrolysis of commercial sago starch

Wee, L.L. and Annuar, M.S.B.M. and Ibrahim, Shaliza (2011) Short communication: energetics of glucoamylase-catalyzed hydrolysis of commercial sago starch. Asia Pacific Journal of Molecular Biology and Biotechnology, 19 (4). pp. 117-120. ISSN 0128-7451,

Preview

Text (Short communication: Energetics of glucoamylase-catalyzed hydrolysis of commercial sago starch) Short_communication_Energetics_of_glucoamylase-catalyzed_hydrolysis_of_commercial_sago_starch.pdf - Published Version Download (488kB)

Abstract

Glucoamylase (EC 3.2.1.3) from Aspergillus niger was used to hydrolyze commercial sago starch into reducing sugars. The energetics of the hydrolysis was studied at optimal substrate concentration, enzyme amount and pH for the process. The ΔH and ΔS estimated via van't Hof analysis were 7.9 J.mole -1 and 6.2 J.mole -1.K -1, respectively. The results indicated that the hydrolysis process is an endothermic reaction and spontaneous within the temperature range tested. It is calculated that 26.3 J.mole -1 of activation energy, E a is required for the enzymatic conversion of sago starch into reducing sugars. The spontaneous reaction with relatively low activation energy supported the viability of glucoamylase-catalyzed hydrolysis of sago starch.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Export Date: 13 February 2014 Source: Scopus Language of Original Document: English Correspondence Address: Annuar, M. S. B. M.; Institute of Biological Sciences, Faculty of Science, Kuala Lumpur 50603, Malaysia; email: sufanannuar@um.edu.my References: Adinarayaan, K., Suren, S., Response surface optimization of enzymatic hydrolysis of maize starch for higher glucose production (2005) Biochemical Engineering Journal, 27, pp. 179-190; Carlos, R.S., Pappy, J.R., Anil, K.P., Woiciechowski, L., Luciana, P.S.V., Ashok, P., (2006), pp. 221-237. , Glucoamylase: In Ashok, P., Colin, W., Carlos, R.S. and Christian, L. (ed), Enzyme technology. India: Springer, Asiatech PublCornish-Bowden, A., (1972) Fundamentals of Enzyme Kinetic, , United Kingdom: Butterworth & Co. (Publ.) Ltd; Miller, G.L., Use of DNS reagent for determination of reducing sugars (1959) Analytical Chemistry, 31, pp. 426-428; Shuler, M.L., Kargi, F., (1992) Bioprocess Engineering: Basic Concepts, , USA: Prentice-Hall International; Silberberg, M.S., (2006) Chemistry: The Molecular Nature of Matter and Change, , 5th edn. New York: McGraw-Hill International, Inc; Singhal, R.S., Kennedy, J.F., Gopalakrishnan, S.M., Kacz-Marek, A., Knill, C.J., Akmar, P.F., Industrial production, processing, and utilization of sago palm-derived products (2008) Carbohydrate Polymers, 72, pp. 1-20; Suraini, A.A., Review: Sago starch and its Utilization (2002) Journal of Bioscience and Bioengineering, 94, pp. 526-529; Stanton, R., Have your trees and eat them (1993) Food Science and Technology Today, 7, pp. 89-94; Swinkels, J.J.M., Sources of starch, its chemistry and physics (1985) Starch Conversion Technology, pp. 15-45. , eds. G. M. A. van Beynum and J. A. Roels, Marcel Dekker, New York; Wang, W.J., Powell, A.D., Oates, C.G., Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes (1996) Bioresource Technology, 55, pp. 55-61; Wee, L.L., Annuar, M.S.M., Ibrahim, S., Chisti, Y., Enzyme-mediated production of sugars from sago starch: Statistical process optimization (2011) Chemical Engineering Communications, 198, pp. 1339-1353
Uncontrolled Keywords:	Energetics, Glucoamylase, Reducing sugars, Sago starch, Aspergillus niger.
Subjects:	T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Engineering
Depositing User:	Mr Jenal S
Date Deposited:	26 Feb 2014 01:55
Last Modified:	11 Feb 2019 07:27
URI:	http://eprints.um.edu.my/id/eprint/9445

Actions (login required)

View Item