Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

Ibrahim, F. and Jahanshahi, P. and Abd Rahman, N. and Abdul Kahar, M.K.B. and Madou, M. and Nozari, A.A. and Soin, N. and Md Dawal, S.Z. and Abi Samra, K. (2010) Analysis and experiment of centrifugal force for microfluidic ELISA CD platform. In: 2010 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2010, 2010, Kuala Lumpur.

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Abstract

This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9 error when compared with the theoretical calculation of burst frequency for all the reservoirs. © 2010 IEEE.

Item Type:	Conference or Workshop Item (Paper)
Funders:	UNSPECIFIED
Additional Information:	Conference code: 84636 Cited By (since 1996):4 Export Date: 29 January 2014 Source: Scopus Art. No.: 5742282 doi: 10.1109/IECBES.2010.5742282 Language of Original Document: English Correspondence Address: Ibrahim, F.; Dept. of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia; email: fatimah@um.edu.my References: Gorkin, R.A., Clime, L., Madou, M.J., Kido, H., Pneumatic pumping in centrifugal microfluidic platforms (2010) Microjluidics and Nanojluidics, 9, pp. 541-549; Siegrist, J., Gorkin, R.A., Bastien, M., Stewart, G., Peytavi, R., Kido, H., Bergeron, M.G., Madou, M.J., Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples (2010) Lab on A Chip, 10, pp. 363-371; Gliere, A., Delattre, C., Modeling and fabrication of capillary stop valves for planar microfluidic systems (2006) Sensors and Actuators, A: Physical, 130-131 (SPEC. ISS.), pp. 601-608. , DOI 10.1016/j.sna.2005.12.011, PII S0924424705007272; Andersson, P., Jesson, G., Kylberg, G., Ekstrand, G., Thorsen, G., Parallel nanoliter microfluidic analysis system (2007) Analytical Chemistry, 79 (11), pp. 4022-4030. , DOI 10.1021/ac061692y; Morais, S., Tamarit-López, J., Carrascosa, J., Puchades, R., Maquieira, A., Analytical prospect of compact disk technology in immunosensing (2008) Analytical and Bioanalytical Chemislly, 391, pp. 2837-2844; He, H., Yuan, Y., Wang, W., Chiou, N., Epstein, A.J., Lee, L.J., (2009) Design and Testing of A Microfluidic Biochip for Cytokine Enzymelinked Immunosorbent Assay, pp. 1-17; Bergeron, M.G., Revolutionizing the practice of medicine through rapid < 1h) DNA-based diagnostics (2008) Clinical and Investigative Medicine. Medecine Clinique et Experimentale, 31, pp. E265-E271; Li, C., Dong, X., Qin, J., Lin, B., Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device (2009) Analytica Chimica Acta, 640, pp. 93-99; Jia, G., Ma, K.-S., Kim, J., Zoval, J.V., Peytavi, R., Bergeron, M.G., Madou, M.J., Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform (2006) Sensors and Actuators, B: Chemical, 114 (1), pp. 173-181. , DOI 10.1016/j.snb.2005.04.043, PII S0925400505004521; Madou, M., Zoval, J., Jia, G., Kido, H., Kim, J., Kim, N., Lab on a CD (2006) Annual Review of Biomedical Engineering, 8, pp. 601-628. , DOI 10.1146/annurev.bioeng.8.061505.095758; Steigert, J., Grumann, M., Dube, M., Streule, W., Riegger, L., Brenner, T., Koltay, P., Ducree, J., Direct hemoglobin measurement on a centrifugal microfluidic platform for point-of-care diagnostics (2006) Sensors and Actuators, A: Physical, 130-131 (SPEC. ISS.), pp. 228-233. , DOI 10.1016/j.sna.2006.01.031, PII S0924424706000719; Riegger, L., Grumann, M., Steigert, J., Lutz, S., Steinert, C.P., Mueller, C., Viertel, J., Ducree, J., Single-step centrifugal hematocrit determination on a 10- processing device (2007) Biomedical Microdevices, 9 (6), pp. 795-799. , DOI 10.1007/s10544-007-9091-1; Kim, N., Dempsey, C.M., Zoval, J.V., Sze, J.-Y., Madou, M.J., Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans (2007) Sensors and Actuators, B: Chemical, 122 (2), pp. 511-518. , DOI 10.1016/j.snb.2006.06.026, PII S0925400506004588; Lafleur, J.P., Salin, E.D., Pre-concentration of trace metals on centrifugal microfluidic discs with direct determination by laser ablation inductively coupled plasma mass spectrometry (2009) Journal of Analytical Atomic Spectromefly, 24, p. 1511; Xi, Y., Templeton, E.J., Salin, E.D., Rapid simultaneous determination of nitrate and nitrite on a centrifugal microfluidic device (2010) Talanta; Xi, Y., Duford, D.A., Salin, E.D., Automated liquid-solid extraction of pyrene from soil on centrifugal microfluidic devices (2010) Talanta, 82, pp. 1072-1076; Chen, Q.L., Ho, H.P., Suen, Y.K., Kong, S.K., Li, W.J., Wong, C., Design and fabrication of centrifugal microfluidic disk for allergic response monitoring (2009) 2009 14th OptoElectronics and Communications Conference, pp. 1-2; Noroozi, Z., Kido, H., Micic, M., Pan, H., Bartolome, C., Princevac, M., Zoval, J., Madou, M.J., Reciprocating flow-based centrifugal microfluidics mixer (2009) The Review of Scientific Instruments, 80, p. 075102; Steigert, J., Haeberle, S., Brenner, T., Muller, C., Steinert, C.P., Koltay, P., Gottschlich, N., Ducree, J., Rapid prototyping of microfluidic chips in COC (2007) Journal of Micromechanics and Microengineering, 17 (2), pp. 333-341. , DOI 10.1088/0960-1317/17/2/020, PII S0960131707288643, 020; Lin, S.I., A novel splitter design for microfluidic biochips using centrifugal driving forces (2010) Microjluidics and Nanojluidics, 9, pp. 523-532; Grumann, M., Brenner, T., Beer, C., Zengerle, R., Ducree, J., Visualization of flow patterning in high-speed centrifugal microfluidics (2005) Review of Scientific Instruments, 76, p. 025101
Uncontrolled Keywords:	Burst frequency, centrifugal force, ELISA, Lab on CD, Microfluidics, microsystem, 2 layer, Capillary force, Centrifugal Forces, Driving forces, Enzyme linked immunosorbent assay, Experimental test, Fluid flow, Sealing films, Spin stand, Substrate layers, Theoretical calculations, Thermal seals, Visualization system, Washing solution, Biomedical engineering, Centrifugation, Experiments, Flow of fluids, Fluid dynamics, Liquids, Microsystems, Visualization
Subjects:	T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Engineering
Depositing User:	Mr Jenal S
Date Deposited:	18 Feb 2014 01:21
Last Modified:	01 Nov 2017 05:32
URI:	http://eprints.um.edu.my/id/eprint/9255

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