Aeromonas dhakensis: Clinical isolates with high carbapenem resistance

Puah, Suat Moi and Khor, Wei Ching and Aung, Kyaw Thu and Lau, Tien Tien Vicky and Puthucheary, S. D. and Chua, Kek Heng (2022) Aeromonas dhakensis: Clinical isolates with high carbapenem resistance. Pathogens, 11 (8). ISSN 2076-0817, DOI

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Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These bla(cphA) were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that A. dhakensis isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights.

Item Type: Article
Funders: Universiti Malaya, Singapore Food Agency
Uncontrolled Keywords: Aeromonas dhakensis; Carbapenem; CLSI; EUCAST; MicroScan
Subjects: Q Science > QR Microbiology
R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Sep 2023 07:05
Last Modified: 21 Sep 2023 07:05

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