Puah, Suat Moi and Khor, Wei Ching and Aung, Kyaw Thu and Lau, Tien Tien Vicky and Puthucheary, S. D. and Chua, Kek Heng (2022) Aeromonas dhakensis: Clinical isolates with high carbapenem resistance. Pathogens, 11 (8). ISSN 2076-0817, DOI https://doi.org/10.3390/pathogens11080833.
Full text not available from this repository.Abstract
Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These bla(cphA) were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that A. dhakensis isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya, Singapore Food Agency |
| Uncontrolled Keywords: | Aeromonas dhakensis; Carbapenem; CLSI; EUCAST; MicroScan |
| Subjects: | Q Science > QR Microbiology R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 21 Sep 2023 07:05 |
| Last Modified: | 21 Sep 2023 07:05 |
| URI: | http://eprints.um.edu.my/id/eprint/41393 |
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