Ternary glycerol-based deep eutectic solvents: Physicochemical properties and enzymatic activity

Rashid, Shahidah Nusailah and Hayyan, Adeeb and Hayyan, Maan and Hashim, Mohd Ali and Elgharbawy, Amal A. M. and Sani, Fazrizatul Shakilla and Basirun, Wan Jefrey and Lee, Vannajan Sanghiran and Alias, Yatimah and Mohammed, Alaa Kareem and Mirghani, Mohamed E. S. and Zulkifli, Mohd Yakub and Rageh, Maher (2021) Ternary glycerol-based deep eutectic solvents: Physicochemical properties and enzymatic activity. Chemical Engineering Research & Design, 169. pp. 77-85. ISSN 0263-8762, DOI https://doi.org/10.1016/j.cherd.2021.02.032.

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The present study investigates deep eutectic solvents (DESs) as potential media for enzymatic hydrolysis. A series of ternary ammonium and phosphonium-based DESs were prepared at different molar ratios by mixing with aqueous glycerol (85%). The physicochemical properties including surface tension, conductivity, density, and viscosity were measured at a temperature range of 298.15 K - 363.15 K. The eutectic points were highly influenced by the variation of temperature. The eutectic point of the choline chloride: glycerol: water (ratio of 1: 2.55: 2.28) and methyltriphenylphosphonium bromide:glycerol:water (ratio of 1: 4.25: 3.75) is 213.4 K and 255.8 K, respectively. The stability of the lipase enzyme isolated from porcine pancreas (PPL) and Rhizopus niveus (RNL) toward hydrolysis in ternary DESs medium was investigated. The PPL showed higher activity compared to the RNL in DESs. Molecular docking simulation of the selected DES with the substrate (p-nitrophenyl palmitate) toward PPL was also reported. It is worth noting that ternary DES systems would be viable lipase activators in hydrolysis reactions. (c) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

Item Type: Article
Funders: University of Malaya RU Geran Fakulti Program [GPF040A2018], University of Malaya Grant Bantuan Kecil Penyelidikan (BKP) [BK033-2017]
Uncontrolled Keywords: Pancreatic lipase; Green solvent; Rhizopus niveus lipase; Ionic liquid; Choline chloride; Molecular docking simulation; Enzymatic hydrolysis
Subjects: T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Academy of Islamic Studies > Dept of Al-Quran & Al- Hadith
Faculty of Engineering > Department of Chemical Engineering
Faculty of Science > Department of Chemistry
Depositing User: Ms Zaharah Ramly
Date Deposited: 14 Sep 2022 08:06
Last Modified: 14 Sep 2022 08:06
URI: http://eprints.um.edu.my/id/eprint/34556

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