Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios

Bonny, Sharmin Quazi and Hossain, M. A. Motalib and Uddin, Syed Muhammad Kamal and Pulingam, Thiruchelvi and Sagadevan, Suresh and Johan, Mohd Rafie (2022) Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios. Critical Reviews in Food Science and Nutrition, 62 (5). pp. 1317-1335. ISSN 1040-8398, DOI

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Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most significant aquatic pathogens of the genera Vibrio, account for most Vibrio-associated outbreaks worldwide. Rapid identification of these pathogens is of great importance for disease surveillance, outbreak investigations and food safety maintenance. Traditional culture dependent methods are time-consuming and labor-intensive whereas culture-independent polymerase chain reaction (PCR) based assays are reliable, consistent, rapid and reproducible. This review covers the recent development and applications of PCR based techniques, which have accelerated advances in the analysis of nucleic acids to identify three major pathogenic vibrios. Emphasis has been given to analytical approaches as well as advantages and limits of the available methods. Overall, this review article possesses the substantial merit to be used as a reference guide for the researchers to develop improved PCR based techniques for the differential detection and quantification of Vibrio species.

Item Type: Article
Funders: Universiti Malaya [ST017-2020], University of Malaya [RU001-2020]
Uncontrolled Keywords: PCR-based detection; Vibrio parahaemolyticus; Vibrio cholera; Vibrio vulnificus; pathogenic; food
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Divisions: Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 25 Apr 2022 07:45
Last Modified: 25 Apr 2022 07:45

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