Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies

Jeyaprakasam, Nantha Kumar and Low, Van Lun and Liew, Jonathan Wee Kent and Pramasivan, Sandthya and Wan-Sulaiman, Wan-Yusoff and Saeung, Atiporn and Vythilingam, Indra (2022) Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies. Scientific Reports, 12 (1). ISSN 2045-2322, DOI

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Blood feeding and host-seeking behaviors of a mosquito play an imperative role in determining its vectorial capacity in transmitting pathogens. Unfortunately, limited information is available regarding blood feeding behavior of Anopheles species in Malaysia. Collection of resting Anopheles mosquitoes for blood meal analysis poses a great challenge especially for forest dwelling mosquitoes. Therefore, a laboratory-based study was conducted to evaluate the potential use of mosquitoes caught using human landing catch (HLC) for blood meal analysis, and subsequently to document blood feeding behavior of local Anopheles mosquitoes in Peninsular Malaysia. The laboratory-based experiment from this study revealed that mosquitoes caught using HLC had the potential to be used for blood meal analysis. Besides HLC, mosquitoes were also collected using manual aspirator and Mosquito Magnet. Overall, 47.4% of 321 field-caught Anopheles mosquitoes belonging to six species were positive for vertebrate host DNA in their blood meal. The most frequent blood meal source was human (45.9%) followed by wild boar (27.4%), dog (15.3%) and monkey (7.5%). Interestingly, only Anopheles cracens and Anopheles introlatus (Leucosphyrus Group) fed on monkey. This study further confirmed that members of the Leucosphyrus Group are the predominant vectors for knowlesi malaria transmission in Peninsular Malaysia mainly due to their simio-anthropophagic feeding behavior.

Item Type: Article
Funders: Ministry of Higher Education of Malaysia Long Term Research Grant Scheme (LRGS) [Grant No: LRGS 1/2018/UM/01/1/3], Ministry of Higher Education, Malaysia, under the Higher Institution Center of Excellence (HICoE) [Grant No: MO002-2019]
Uncontrolled Keywords: Blood feeding; Mosquito; Anopheles species; Simio-anthropophagic
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Divisions: Faculty of Medicine
Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 29 Jul 2022 08:34
Last Modified: 29 Jul 2022 08:34

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