Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa

Syuhada, Nur Hidayu and Merican, Faradina and Zaki, Syazana and Broady, Paul A. and Convey, Peter and Muangmai, Narongrit (2022) Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa. Scientific Reports, 12 (1). ISSN 2045-2322, DOI

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This study was initiated following the serendipitous discovery of a unialgal culture of a Stichococcus-like green alga (Chlorophyta) newly isolated from soil collected on Signy Island (maritime Antarctica) in growth medium supplemented with 100 mu g/mL cycloheximide (CHX, a widely used antibiotic active against most eukaryotes). In order to test the generality of CHX resistance in taxa originally identified as members of Stichococcus (the detailed taxonomic relationships within this group of algae have been updated since our study took place), six strains were studied: two strains isolated from recent substrate collections from Signy Island (maritime Antarctica) (''Antarctica'' 1 and ``Antarctica'' 2), one isolated from this island about 50 years ago (''Antarctica'' 3) and single Arctic (''Arctic''), temperate (''Temperate'') and tropical (''Tropical'') strains. The sensitivity of each strain towards CHX was compared by determining the minimum inhibitory concentration (MIC), and growth rate and lag time when exposed to different CHX concentrations. All strains except ``Temperate'' were highly resistant to CHX (MIC > 1000 mu g/mL), while ``Temperate'' was resistant to 62.5 mu g/mL (a concentration still considerably greater than any previously reported for algae). All highly resistant strains showed no significant differences in growth rate between control and treatment (1000 mu g/mL CHX) conditions. Morphological examination suggested that four strains were consistent with the description of the species Stichococcus bacillaris while the remaining two conformed to S. mirabilis. However, based on sequence analyses and the recently available phylogeny, only one strain, ``Temperate'', was confirmed to be S. bacillaris, while ``Tropical'' represents the newly erected genus Tetratostichococcus, ``Antarctica 1'' Tritostichococcus, and ``Antarctica 2'', ``Antarctica 3'' and ``Arctic'' Deuterostichococcus. Both phylogenetic and CHX sensitivity analyses suggest that CHX resistance is potentially widespread within this group of algae.

Item Type: Article
Funders: YPASM Berth Support, YPASM Fellowship [Grant No: 304/PBIOLOGI/650963], RUI grant [Grant No: 1001/PBIOLOGI/811305 ], Habitat Foundation research grant, UK Research & Innovation (UKRI) Natural Environment Research Council (NERC), Universiti Sains Malaysia
Uncontrolled Keywords: Unialgal culture; Antibiotic-resistance; Antimicrobial activity; Bacteria
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 01 Aug 2022 02:49
Last Modified: 01 Aug 2022 02:49

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