Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Teoh, Boon Yew and Lim, Yang Mooi and Chong, Wu Yi and Subramaniam, Menaga and Tan, Zi Zhang and Misran, Misni and Suk, Vicit Rizal Eh and Lo, Kwok-Wai and Lee, Poh Foong (2022) Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel. Biomedical Microdevices, 24 (1). ISSN 1387-2176, DOI

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Isolation of exosome from culture medium in an effective way is desired for a less time consuming, cost saving technology in running the diagnostic test on cancer. In this study, we aim to develop an inertial microfluidic channel to separate the nano-size exosome from C666-1 cell culture medium as a selective sample. Simulation was carried out to obtain the optimum flow rate for determining the dimension of the channels for the exosome separation from the medium. The optimal dimension was then brought forward for the actual microfluidic channel fabrication, which consisted of the stages of mask printing, SU8 mould fabrication and ended with PDMS microchannel curing process. The prototype was then used to verify the optimum flow rate with polystyrene particles for its capabilities in actual task on particle separation as a control outcome. Next, the microchip was employed to separate the selected samples, exosome from the culture medium and compared the outcome from the conventional exosome extraction kit to study the level of effectiveness of the prototype. The exosome outcome from both the prototype and extraction kits were characterized through zetasizer, western blot and Transmission electron microscopy (TEM). The microfluidic chip designed in this study obtained a successful separation of exosome from the culture medium. Besides, the extra benefit from this microfluidic channels in particle separation brought an evenly distributed exosome upon collection while the exosomes separated through extraction kit was found clustered together. Therefore, this work has shown the microfluidic channel is suitable for continuous separation of exosome from the culture medium for a clinical study in the future.

Item Type: Article
Funders: UTARRF [IPSR/RMC/UTARRF/2017-C2/T07], University of Malaya Research grant [RU013-2018]
Additional Information: RS
Uncontrolled Keywords: Microfluidic; Exosome separation; Nasopharangeal cancer; Continuous Separation
Subjects: Q Science > QC Physics
R Medicine > R Medicine (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Science > Department of Chemistry
Deputy Vice Chancellor (Research & Innovation) Office > Photonics Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 10 Aug 2022 01:41
Last Modified: 10 Aug 2022 01:41

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