Antioxidant and cytotoxicity activities of butylated hydroxytoluene ligands capped gold nanoparticles

Ahmad, Mohd Hafiz and Yehye, Wageeh A. and Abd Rahman, Noorsaadah and Al-Ani, Lina A. and Johan, Mohd Rafie and Lu, Jun and Hashim, Najihah M. (2021) Antioxidant and cytotoxicity activities of butylated hydroxytoluene ligands capped gold nanoparticles. Chiang Mai Journal of Science, 48 (2). pp. 405-419. ISSN 0125-2526,

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The new potent nano-antioxidant of sulfur-containing butylated hydroxytoluene ligands (S-BHTLs)-conjugated with gold nanoparticles, Au-S-BHTLs, was synthesized by a conjugation of sulfur-containing ligands derived from BHT on the surface of gold nanoparticles (AuNPs). The in-house developing eight sulfur-containing BHT-ligands (S-BHTLs) were used for further study on functionalization with AuNPs and their biological activities. The antioxidant and cytotoxic abilities of eight types of Au-S-BHTLs such as compound Au-2a, Au-2b, Au-3a, Au-3b, Au-4a, Au-4b, Au-5a and Au-5b were tested by DPPH center dot(2,2-diphenyl-1-picrylhydrazyl) radical scavenging and MTT assays against HT29 (human colorectal adenocarcinoma) and MCF7 (human breast adenocarcinoma). Based on the results, it was revealed that nanocomposites Au-5a and Au-3a attained over 50% lower IC50 values against free radical than the un-functionalized AuNPs. Meanwhile, nanocomposite Au-2b showed the highest cytotoxicity effect against both cancer cell lines. Selectivity and safety towards non-tumorgenic cell lines were also evaluated and proved superior selectivity index (SI) of all nanocomposites (SI > 2.00) against both cancer cells except Au-5b (SI = 1.87) against MCF7. Hence, the functionalization of S-BHTLs with gold nanoparticles has increased the selectivity of nanocomposites and enhanced the antioxidant potentials to become useful and promising anticancer agents.

Item Type: Article
Funders: Ministry of Education, Malaysia (FP050-2014B) (FP127-2019A), Universiti Malaya (PPP-2015B)
Uncontrolled Keywords: Gold nanoparticles; Au-S-BHTLs; DPPH; HT29; MCF7
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Depositing User: Ms Zaharah Ramly
Date Deposited: 01 Mar 2022 00:57
Last Modified: 01 Mar 2022 00:57

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