DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations

Khalil, Ibrahim and Hashem, Abu and Nath, Amit R. and Julkapli, Nurhidayatullaili Muhd and Yehye, Wageeh A. and Basirun, Wan Jeffrey (2021) DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations. Molecular and Cellular Probes, 59. ISSN 0890-8508, DOI https://doi.org/10.1016/j.mcp.2021.101758.

Full text not available from this repository.


Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity, sensitivity and unsuitability for processed food products or complex food matrices. On the other hand, DNA based molecular techniques and nanoparticle based DNA biosensing strategies are gathering huge attention from the scientific communities, researchers and are considered as one of the best alternatives to the conventional strategies. Though nucleic acid based molecular techniques such as PCR and DNA sequencing are getting greater successes in species detection, they are still facing problems from its point-of-care applications. In this context, nanoparticle based DNA biosensors have gathered successes in some extent but not to a satisfactory stage to mark with. In recent years, many articles have been published in the area of progressive nucleic acid-based technologies, however there are very few review articles on DNA nanobiosensors in food science and technology. In this review, we present the fundamentals of DNA based molecular techniques such as PCR, DNA sequencing and their applications in food science. Moreover, the in-depth discussions of different DNA biosensing strategies or more specifically electrochemical and optical DNA nanobiosensors are presented. In addition, the significance of DNA nanobiosensors over other advanced detection technologies is discussed, focusing on the deficiencies, advantages as well as current challenges to ameliorate with the direction for future development.

Item Type: Article
Funders: University Malaya Research Grant (UMRG RP044A-17AET)
Uncontrolled Keywords: Biosensors; DNA; Electrochemical; Nanomaterials; Optical; PCR
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > R Medicine (General) > Medical technology
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms Zaharah Ramly
Date Deposited: 29 Mar 2022 08:07
Last Modified: 29 Mar 2022 08:07
URI: http://eprints.um.edu.my/id/eprint/28280

Actions (login required)

View Item View Item