The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia

Mobasseri, Golnaz and Teh, Cindy Shuan Ju and Ooi, Peck Toung and Thong, Kwai Lin (2019) The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia. Journal of Global Antimicrobial Resistance, 17. pp. 227-232. ISSN 2213-7165, DOI

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Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Malaysia. Methods: A total of 46 swine K. pneumoniae strains isolated from 2013–2015 in Malaysia were analysed for the production of extended-spectrum β-lactamases and carbapenemase. The resistance traits and genetic diversity of these strains were characterised by polymerase chain reaction, conjugation, plasmid analysis, and pulsed-field gel electrophoresis. Results: Nineteen of 46 strains were multidrug resistant while 13 were resistant to colistin. The majority of colistin-resistant strains harboured blaTEM gene (92.3%), followed by blaSHV (69.23%), blaCTXM-1 (38.46%), and blaMCR-1 (23.08%). All three colistin-resistant strains had transferable plasmids and the colistin resistance gene blaMCR-1. Genotyping by pulsed-field gel electrophoresis showed high genetic diversity among the K. pneumoniae and that the colistin-resistant K. pneumoniae strains were heterogenous. Conclusion: It is believed that this is the first report of colistin-resistant K. pneumoniae among swine strains associated with mcr-1 plasmid in Malaysia. Due to the emergence of β-lactam, carbapenem and colistin resistance, the use of colistin in animal husbandry and agriculture should be avoided to prevent treatment failure. © 2019 International Society for Chemotherapy of Infection and Cancer

Item Type: Article
Funders: University of Malaya Postgraduate Research Grant — ( PG072-2014B ), High Impact Research (HIR) Grant ( UM.C/625/1/HIR/MOHE/CHAN/11/02 )
Uncontrolled Keywords: Colistin; Genetic diversity; Klebsiella pneumoniae; Multidrug resistance; Swine
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 12 Mar 2020 02:20
Last Modified: 12 Mar 2020 02:20

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