Mobasseri, Golnaz and Teh, Cindy Shuan Ju and Ooi, Peck Toung and Thong, Kwai Lin (2019) The emergence of colistin-resistant Klebsiella pneumoniae strains from swine in Malaysia. Journal of Global Antimicrobial Resistance, 17. pp. 227-232. ISSN 2213-7165, DOI https://doi.org/10.1016/j.jgar.2018.12.015.
Full text not available from this repository.Abstract
Objective: Colistin is the last line of therapy for infections caused by multidrug-resistant Gram-negative bacteria. The objective of this study was to determine the phenotypic and genotypic characteristics of colistin-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from swine samples in Malaysia. Methods: A total of 46 swine K. pneumoniae strains isolated from 2013–2015 in Malaysia were analysed for the production of extended-spectrum β-lactamases and carbapenemase. The resistance traits and genetic diversity of these strains were characterised by polymerase chain reaction, conjugation, plasmid analysis, and pulsed-field gel electrophoresis. Results: Nineteen of 46 strains were multidrug resistant while 13 were resistant to colistin. The majority of colistin-resistant strains harboured blaTEM gene (92.3%), followed by blaSHV (69.23%), blaCTXM-1 (38.46%), and blaMCR-1 (23.08%). All three colistin-resistant strains had transferable plasmids and the colistin resistance gene blaMCR-1. Genotyping by pulsed-field gel electrophoresis showed high genetic diversity among the K. pneumoniae and that the colistin-resistant K. pneumoniae strains were heterogenous. Conclusion: It is believed that this is the first report of colistin-resistant K. pneumoniae among swine strains associated with mcr-1 plasmid in Malaysia. Due to the emergence of β-lactam, carbapenem and colistin resistance, the use of colistin in animal husbandry and agriculture should be avoided to prevent treatment failure. © 2019 International Society for Chemotherapy of Infection and Cancer
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Postgraduate Research Grant — ( PG072-2014B ), High Impact Research (HIR) Grant ( UM.C/625/1/HIR/MOHE/CHAN/11/02 ) |
| Uncontrolled Keywords: | Colistin; Genetic diversity; Klebsiella pneumoniae; Multidrug resistance; Swine |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history R Medicine |
| Divisions: | Faculty of Medicine Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Mar 2020 02:20 |
| Last Modified: | 12 Mar 2020 02:20 |
| URI: | http://eprints.um.edu.my/id/eprint/24021 |
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