Design and validation of a bi-axial loading bioreactor for mechanical stimulation of engineered cartilage

Yusoff, N. and Abu Osman, Noor Azuan and Pingguan-Murphy, Belinda (2011) Design and validation of a bi-axial loading bioreactor for mechanical stimulation of engineered cartilage. Medical Engineering & Physics, 33 (6). pp. 782-788. ISSN 1350-4533 , DOI https://doi.org/10.1016/j.medengphy.2011.01.013.

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Abstract

A mechanical-conditioning bioreactor has been developed to provide bi-axial loading to three-dimensional (3D) tissue constructs within a highly controlled environment. The computer-controlled bioreactor is capable of applying axial compressive and shear deformations, individually or simultaneously at various regimes of strain and frequency. The reliability and reproducibility of the system were verified through validation of the spatial and temporal accuracy of platen movement, which was maintained over the operating length of the system. In the presence of actual specimens, the system was verified to be able to deliver precise bi-axial load to the specimens, in which the deformation of every specimen was observed to be relatively homogeneous. The primary use of the bioreactor is in the culture of chondrocytes seeded within an agarose hydrogel while subjected to physiological compressive and shear deformation. The system has been designed specifically to permit the repeatable quantification and characterisation of the biosynthetic activity of cells in response to a wide range of short and long term multi-dimensional loading regimes. (C) 2011 IPEM. Published by Elsevier Ltd. All rights reserved.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Yusoff, Norwahida Abu Osman, Noor Azuan Pingguan-Murphy, Belinda eng Evaluation Studies Research Support, Non-U.S. Gov't England 2011/03/02 06:00 Med Eng Phys. 2011 Jul;33(6):782-8. Epub 2011 Feb 26.
Uncontrolled Keywords:	Bioreactors, Cartilage/*physiology, Cartilage, Articular/physiology, Chondrocytes/cytology/*physiology, Compressive Strength, Equipment Design, Finite Element Analysis, Humans, Models, Biological, Physical Stimulation/instrumentation/*methods, Reproducibility of Results, Shear Strength, Stress, Mechanical, Tissue Engineering/instrumentation/*methods.
Subjects:	T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Engineering
Depositing User:	Mr Jenal S
Date Deposited:	14 Jan 2013 01:17
Last Modified:	07 Feb 2019 08:07
URI:	http://eprints.um.edu.my/id/eprint/4413

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