Abdullah, Nur Raihan and Mohd Nasir, Mohd Hamzah and Azizan, Nur Hafizah and Wan Mohtar, Wan Abd Al Qadr Imad and Sharif, Faez (2022) Bioreactor-grown exo- and endo-beta-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment. Frontiers in Bioengineering And Biotechnology, 10. ISSN 2296-4185, DOI https://doi.org/10.3389/fbioe.2022.960320.
Full text not available from this repository.Abstract
This study aims to identify the roles of exo-beta-glucan (EPS- BG) and endo-beta-glucan (ENS-BG) extracted from Ganoderma lucidum (GL) in inhibiting the alpha-glucosidase enzyme, a target mechanism for postprandial hyperglycaemia regulation. Upscale production of GL was carried out using a 10 L bioreactor. The zebrafish embryo toxicity test (ZFET) was carried out based on OECD guidelines. The hatching rate, survival rate, heart rate, morphological malformation, and teratogenic defects were observed and determined every 24 h from 0-120 h of post-exposure (hpe). For diabetes induction, adult zebrafish (3-4 months of age) were overfed and induced with three doses of 350 mg/kg streptozotocin (STZ) by intraperitoneal injection (IP) on three different days (days 1, 3, and 5). The oral sucrose tolerance test (OSTT) and anti-diabetic activity of EPS-BG and ENS-BG were evaluated (day 7) using the developed model (n = 15). This study showed that EPS is the most potent compound with the highest inhibitory effect toward the alpha-glucosidase enzyme with an IC50 value of 0.1575 mg/ml compared to ENS extracts (IC50 = 0.3479 mg/ml). Both EPS-BG and ENS-BG demonstrated a strong inhibition of alpha-glucosidase activity similar to the clinically approved alpha-glucosidase inhibitor, acarbose (IC50 = 0.8107 mg/ml). ENS- BG is non-toxic toward zebrafish embryos with LC50 of 0.92 mg/ml and showed no significant changes in ZE hatching and normal heart rate as compared to untreated embryos (161 beats/min). Teratogenic effects of ENS-BG (<1.0 mg/ml) on zebrafish embryonic development were not observed. The DM model of zebrafish was acquired after the third dose of STZ with a fasting BGL of 8.98 +/- 0.28 mmol/L compared to the normal healthy group (4.23 +/- 0.62 mmol/L). The BGL of DM zebrafish after 30 min treated with EPS-BG and ENS-BG showed a significant reduction (p < 0.0001). Both EPS-BG and ENS-BG significantly reduced DM zebrafish's peak blood glucose and the area under the curve (AUC) in OSTT. Hence, EPS-BG and ENS-BG extracted from GL showed promising inhibition of the alpha-glucosidase enzyme and are considered non-toxic in ZE. Moreover, EPS-BG and ENS-BG reduced blood glucose levels and inhibited hyperglycemia in DM zebrafish.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia [FRGS/1/2019/STG04/UIAM/03/1] |
| Uncontrolled Keywords: | Ganoderma lucidum; Alpha-glucosidase; Polysaccharide; Embryo toxicity test; Zebrafish |
| Subjects: | Q Science > Q Science (General) T Technology > T Technology (General) |
| Divisions: | Faculty of Science |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Sep 2023 02:29 |
| Last Modified: | 12 Sep 2023 02:29 |
| URI: | http://eprints.um.edu.my/id/eprint/41168 |
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