Bioassay-guided isolation of cytotoxic cycloartane triterpenoid glycosides from the traditionally used medicinal plant Leea Indica

Wong, Yau Hsiung and Kadir, Habsah Abdul and Ling, Sui Kiong (2012) Bioassay-guided isolation of cytotoxic cycloartane triterpenoid glycosides from the traditionally used medicinal plant Leea Indica. Evidence-Based Complementary and Alternative Medicine, 2012. p. 164689. ISSN 1741-427X, DOI PMID: 22203865.

Preview

PDF 30.pdf Download (3MB)

Abstract

Leea indica is a medicinal plant used traditionally to cure cancer. In this study, the cytotoxic compounds of L. indica were isolated using bioassay-guided approach. Two cycloartane triterpenoid glycosides, mollic acid arabinoside (MAA) and mollic acid xyloside (MAX), were firstly isolated from L. indica. They inhibited the growth of Ca Ski cervical cancer cells with IC(50) of 19.21 μM (MAA) and 33.33 μM (MAX). MRC5 normal cell line was used to calculate selectivity index. MAA and MAX were about 8 and 4 times more cytotoxic to Ca Ski cells compared to MRC5. The cytotoxicity of MAA was characterized by both cytostatic and cytocidal effects. MAA decreased the expression of proliferative cell nuclear antigen, increased sub-G1 cells, and arrested cells in S and G2/M phases. This study provides the evidence for the ethnomedicinal use of L. indica and paves the way for future mechanism studies on the anticancer effects of MAA.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Biomolecular Research Group, Biochemistry Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ---> Correspondence should be addressed to Habsah Abdul Kadir, aakhak@yahoo.com
Uncontrolled Keywords:	Leea Indica; Biochemistry
Subjects:	R Medicine
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Mr. Faizal Hamzah
Date Deposited:	04 Jul 2012 06:22
Last Modified:	29 Jan 2020 06:01
URI:	http://eprints.um.edu.my/id/eprint/3471

Actions (login required)

View Item