Proteolytic profile, angiotensin-I converting enzyme inhibitory activity and sensory evaluation of Codonopsis pilosula and fish collagen cheese

Shori, Amal Bakr and Hong, Yoong Chia and Baba, Ahmad Salihin (2021) Proteolytic profile, angiotensin-I converting enzyme inhibitory activity and sensory evaluation of Codonopsis pilosula and fish collagen cheese. Food Research International, 143. ISSN 0963-9969, DOI https://doi.org/10.1016/j.foodres.2021.110238.

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Abstract

Four types of cheeses were prepared included plain- cheese (control), Codonopsis pilosula (CP)- cheese, plaincheese with fish collagen (FC; control) and CP- cheese with FC. The effects of cheese samples on acidification, proteolysis of milk proteins using three methods (cadmium-ninhydrin method, O-phthaldialdehyde (OPA) assay, and electrophoresis assay), and angiotensin-converting enzyme (ACE)-inhibitory activity were investigated during 0, 2, & 4 weeks of ripening. In addition, the sensory evaluation was also investigated during 0, 2, 4, & 8 weeks of ripening. The presence of FC in CP- cheese increased the numbers of free amino acids (FAA) at 0 and 2 weeks. The addition of CP both in the presence and absence of FC affected positively (p < 0.05) on the concentrations of OPA peptide in cheese compared to their respective controls. The presence of CP and/or FC in cheese increased the degradation of milk proteins (alpha-, beta-, & kappa- caseins, beta-lactoglobulin, and alpha-lactalbumin) compared to their respective controls during ripening periods. The highest ACE inhibitory activity was shown at 4 weeks of ripening for CP- cheese both in the absence (67.75 +/- 14.15%) and the presence (78.65 +/- 2.85%) of FC. In addition, 8-week-old CP-cheese in the presence and absence of fish collagen had similar organoleptic characteristics to plain-cheese. In conclusion, C. pilosula and/or fish collagen may lead to the development in the production and formulation of cheese with anti-ACE activity.

Item Type: Article
Funders: Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah (D-312-247-1440), DSR
Uncontrolled Keywords: Cheese; Codonopsis pilosula; Proteolysis; Fish collagen; ACE inhibitory activity
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
T Technology > T Technology (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 14 May 2022 06:52
Last Modified: 14 May 2022 06:52
URI: http://eprints.um.edu.my/id/eprint/27054

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