Ali, M.E. and Razzak, M.A. and Abd Hamid, S.B. and Rahman, M.M. and Al Amin, M. and Abd Rashid, N.R. and Asing, - (2015) Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods. Food Chemistry, 177. pp. 214-224.
Full text not available from this repository.Abstract
Food falsification has direct impact on public health, religious faith, fair-trades and wildlife. For the first time, here we described a multiplex polymerase chain reaction assay for the accurate identification of five meat species forbidden in Islamic foods in a single assay platform. Five pairs of species-specific primers were designed targeting mitochondrial ND5, ATPase 6, and cytochrome b genes to amplify 172, 163, 141, 129 and 108 bp DNA fragments from cat, dog, pig, monkey and rat meats, respectively. All PCR products were identified in gel-images and electrochromatograms obtained from Experion Bioanalyzer. Species-specificity checking against 15 important meat and fish and 5 plant species detected no cross-species amplification. Screening of target species in model and commercial meatballs reflected its application to detect target species in process foods. The assay was tested to detect 0.01-0.02 ng DNA under raw states and 1% suspected meats in meatball formulation. (C) 2015 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Islamic foods; Multiplex PCR; Halal and haram species; Fair-trade economy |
| Subjects: | B Philosophy. Psychology. Religion > BP Islam. Bahaism. Theosophy, etc > Islam Q Science > Q Science (General) |
| Depositing User: | Mr Faizal 2 |
| Date Deposited: | 13 Apr 2015 02:43 |
| Last Modified: | 13 Apr 2015 02:43 |
| URI: | http://eprints.um.edu.my/id/eprint/13246 |
Actions (login required)
 |
View Item |
