A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida

Annuar, M.S.M. and Tan, I.K.P. and Ibrahim, S. and Ramachandran, K.B. (2008) A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida. Brazilian Journal of Chemical Engineering, 25 (2). pp. 217-228. ISSN 0104-6632

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Abstract

A kinetic model is presented giving a mathematical description of batch culture of Pseudomonas putida PGA1 grown using saponified palm kernel oil as carbon source and ammonium as the limiting nutrient. The growth of the micro-organism is well-described using Tessier-type model which takes into account the inhibitory effect of ammonium at high concentrations. The ammonium consumption rate by the cells is related in proportion to the rate of growth. The intracellular production of medium-chain-length poly-(3-hydroxyalkanoates) (PHAMCL) by P. putida PGA1 cells is reasonably modeled by the modified Luedeking-Piret kinetics, which incorporate a function of product synthesis inhibition (or reduction) by ammonium above a threshold level.

Item Type: Article
Additional Information: 307HK Times Cited:6 Cited References Count:19
Uncontrolled Keywords: Ammonium, Kinetic, Medium-chain-length PHA, P. Putida, Substrate-inhibition, Batch cell culture, Biochemical engineering, Biochemistry, Cell culture, Chain length, Kinetic theory, Batch cultures, Carbon sources, Chain lengths, Consumption rates, H igh concentrations, Hydroxyalkanoates (PHA), Inhibitory effects, Intracellular production, Kinetic modelling, Mathematical descriptions, Palm kernel oil (PKO), Product synthesis, Pseudomonas putida, Threshold levels, Ammonium compounds
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 26 Feb 2014 02:05
Last Modified: 03 Jul 2017 09:04
URI: http://eprints.um.edu.my/id/eprint/9429

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