Electrospun biopolyesters as drug screening platforms for corneal keratocytes

Azari, P. and Luan, N.S. and Gan, S.N. and Yahya, R. and Wong, C.S. and Chua, K.H. and Pingguan-Murphy, B. (2015) Electrospun biopolyesters as drug screening platforms for corneal keratocytes. International Journal of Polymeric Materials and Polymeric Biomaterials, 64 (15). pp. 785-791. ISSN 0091-4037

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Abstract

In vitro drug screening techniques provide rapid and easy to analyze data, while saving a lot of animals from being sacrificed. An important part of any in vitro drug screening platform is a biomaterial which promotes cell growth and proliferation. The potential of electrospun scaffolds made of polyhydroxybutyrate (PHB), poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV), and polycaprolactone (PCL) were studied to serve as drug screening platform for corneal keratocyte tissues. The results showed that the proliferation rate was slightly higher for PCL and PHBV on day 7. Gene expression results showed that PCL was the best in maintaining keratocyte genes. GRAPHICS .

Item Type: Article
Additional Information: ISI Document Delivery No.: CL3TW Times Cited: 0 Cited Reference Count: 29 Cited References: Abadi FJH, 2014, INT J POLYM MATER PO, V63, P57, DOI 10.1080/00914037.2013.769248 Aghdam RM, 2014, INT J POLYM MATER PO, V63, P229, DOI 10.1080/00914037.2013.800983 Chen Y, 2013, PROG RETIN EYE RES, V33, P28, DOI 10.1016/j.preteyeres.2012.10.001 Cui WG, 2007, J APPL POLYM SCI, V103, P3105, DOI 10.1002/app.25464 Cui WG, 2006, BIOMACROMOLECULES, V7, P1623, DOI 10.1021/bm060057z Cui WG, 2008, POLYM DEGRAD STABIL, V93, P731, DOI 10.1016/j.polymdegradstab.2007.12.002 Ghafar NA, 2007, TISSUE ENG REGEN MED, V4, P557 Ghasemi-Mobarakeh L, 2014, INT J POLYM MATER PO, V63, P240, DOI 10.1080/00914037.2013.830247 Guan DH, 2008, APPL SURF SCI, V255, P324, DOI 10.1016/j.apsusc.2008.06.093 Gunatillake PA, 2003, EUR CELLS MATER, V20, P1 Ito Y, 2005, J BIOSCI BIOENG, V100, P43, DOI 10.1263/jbb.100.43 Jester JV, 2003, EXP EYE RES, V77, P581, DOI 10.1016/S0014-4835(03)00188-X JESTER JV, 1995, INVEST OPHTH VIS SCI, V36, P809 KIRKPATRICK CJ, 1990, J MATER SCI-MATER M, V1, P9, DOI 10.1007/BF00705347 Michelacci YM, 2003, BRAZ J MED BIOL RES, V36, P1037, DOI 10.1590/S0100-879X2003000800009 Nikitovic D, 2008, IUBMB LIFE, V60, P818, DOI 10.1002/iub.131 Piersma AH, 2004, TOXICOL LETT, V149, P147, DOI 10.1016/j.toxlet.2003.12.029 Ritchey ER, 2011, MOL VIS, V17, P2440 Saika S, 2000, J BIOL CHEM, V275, P2607, DOI 10.1074/jbc.275.4.2607 Shenoy SL, 2005, POLYMER, V46, P3372, DOI 10.1016/j.polymer.2005.03.011 Sombatmankhong K, 2007, POLYMER, V48, P1419, DOI 10.1016/j.polymer.2007.01.014 Tehrani AH, 2010, J MATER SCI-MATER M, V21, P2989, DOI 10.1007/s10856-010-4149-7 Tomasek JJ, 2002, NAT REV MOL CELL BIO, V3, P349, DOI 10.1038/nrm809 Wang LK, 2007, INT SER DEV HEAT TRA, P27 West-Mays JA, 2006, INT J BIOCHEM CELL B, V38, P1625, DOI 10.1016/j.biocel.2006.03.010 Whitcher JP, 2001, B WORLD HEALTH ORGAN, V79, P214 Wilson S. E., 1998, EYE CONTACT LENS, V24, P181 Wilson SE, 2003, AM J OPHTHALMOL, V136, P530, DOI 10.1016/S0002-9394(02)00085-0 Abidin FZ, 2011, BMC COMPLEM ALTERN M, V11, DOI 10.1186/1472-6882-11-94 Azari, Pedram Luan, Ng Sook Gan, Seng Neon Yahya, Rosiyah Wong, Chiow San Chua, Kien Hui Pingguan-Murphy, Belinda Engineering, Faculty /I-7935-2015; Wong, C.S./C-1047-2010 Engineering, Faculty /0000-0002-4848-7052; Wong, C.S./0000-0002-7186-2578 University of Malaya PV-107-2012A; Ministry of Education Malaysia UM.C/HIR/MOHE/ENG/44 This study was supported by High Impact Research Grant (UM.C/HIR/MOHE/ENG/44) from the Ministry of Education Malaysia and Postgraduate Research Grant (PPP) (PV-107-2012A), University of Malaya. 0 TAYLOR & FRANCIS AS OSLO INT J POLYM MATER PO
Uncontrolled Keywords: Biopolyesters, corneal keratocytes, electrospinning, polycaprolactone, polyhydroxybutyrate, polyhydroxybutyrate-co-hydroxyvalerate, extracellular-matrix, scaffolds, cells, mats, differentiation, regeneration, degradation, lumican, culture, system,
Subjects: T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 04 Apr 2016 00:47
Last Modified: 04 Apr 2016 00:47
URI: http://eprints.um.edu.my/id/eprint/15740

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