Hosseini, S. and Azari, P. and Farahmand, E. and Gan, S.N. and Rothan, H.A. and Yusof, R. and Koole, L.H. and Djordjevic, I. and Ibrahim, F. (2015) Polymethacrylate coated electrospun PHB fibers: An exquisite outlook for fabrication of paper-based biosensors. Biosensors and Bioelectronics, 69. pp. 257-264. ISSN 0956-5663, DOI https://doi.org/10.1016/j.bios.2015.02.034.
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Abstract
Electrospun polyhydroxybutyrate (PHB) fibers were dip-coated by polymethyl methacrylate-co-methacrylic acid, poly(MMA-co-MAA), which was synthesized in different molar ratios of the monomers via free-radical polymerization. Fabricated platfrom was employed for immobilization of the dengue antibody and subsequent detection of dengue enveloped virus in enzyme-linked immunosorbent assay (ELISA). There is a major advantage for combination of electrospun fibers and copolymers. Fiber structre of electrospun PHB provides large specific surface area available for biomolecular interaction. In addition, polymer coated parts of the platform inherited the premanent presence of surface carboxyl (-COOH) groups from MAA segments of the copolymer which can be effectively used for covalent and physical protein immobilization. By tuning the concentration of MAA monomers in polymerization reaction the concentration of surface -COOH groups can be carefully controlled. Therefore two different techniques have been used for immobilization of the dengue antibody aimed for dengue detection: physical attachment of dengue antibodies to the surface and covalent immobilization of antibodies through carbodiimide chemistry. In that perspective, several different characterization techniques were employed to investigate the new polymeric fiber platform such as scanning electron microscopy (SEM), atomic force microscopy (AFM), water contact angle (WCA) measurement and UV-vis titration. Regardless of the immobilization techniques, substantially higher signal intensity was recorded from developed platform in comparison to the conventional ELISA assay. (C) 2015 Elsevier B.V. All rights reserved.
| Item Type: | Article |
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| Funders: | University of Malaya Flagship Grant FL001-14AET , Ministry of Higher Education Malaysia C/625/1/HIR/MOHE/05 , University of Malaya Research Grant UMRG: RP009E-13AET |
| Additional Information: | ISI Document Delivery No.: CL0OV Times Cited: 0 Cited Reference Count: 30 Cited References: Alcon S, 2002, J CLIN MICROBIOL, V40, P376, DOI 10.1128/JCM.40.2.376-381.2002 Bessoff K, 2008, CLIN VACCINE IMMUNOL, V15, P1513, DOI 10.1128/CVI.00140-08 Chantasirichot S, 2012, BIOSENS BIOELECTRON, V38, P209, DOI 10.1016/j.bios.2012.05.029 Chen JP, 2009, J MEMBRANE SCI, V340, P9, DOI 10.1016/j.memsci.2009.05.002 Cipitria A, 2011, J MATER CHEM, V21, P9419, DOI 10.1039/c0jm04502k Coad BR, 2013, SURF COAT TECH, V233, P169, DOI 10.1016/j.surfcoat.2013.05.019 Djordjevic I, 2010, J BIOMAT SCI-POLYM E, V21, P237, DOI 10.1163/156856209X415558 Hong CC, 2013, BIOSENS BIOELECTRON, V50, P425, DOI 10.1016/j.bios.2013.07.016 Hosseini S, 2014, EUR POLYM J, V60, P14, DOI 10.1016/j.eurpolymj.2014.08.010 Hosseini S, 2014, ANALYST, V139, P2933, DOI 10.1039/c3an01789c Hosseini S, 2014, APPL SURF SCI, V300, P43, DOI 10.1016/j.apsusc.2014.01.203 Hosseini S, 2014, APPL SURF SCI, V317, P630, DOI 10.1016/j.apsusc.2014.08.167 Li XJ, 2014, BIOSENS BIOELECTRON, V51, P261, DOI 10.1016/j.bios.2013.07.008 Lin TW, 2010, BIOSENS BIOELECTRON, V25, P2706, DOI 10.1016/j.bios.2010.04.041 Linares EM, 2013, BIOSENS BIOELECTRON, V41, P180, DOI 10.1016/j.bios.2012.08.005 Lu WB, 2014, BIOSENS BIOELECTRON, V57, P219, DOI 10.1016/j.bios.2014.02.027 Luo YL, 2010, BIOSENS BIOELECTRON, V26, P1612, DOI 10.1016/j.bios.2010.08.028 Ma ZW, 2006, J MEMBRANE SCI, V272, P179, DOI 10.1016/j.memsci.2005.07.038 Nie XM, 2014, BIOSENS BIOELECTRON, V58, P314, DOI 10.1016/j.bios.2014.03.007 Sam S., 2009, LANGMUIR, V26, P809, DOI DOI 10.1021/LA902220A SANO S, 1993, BIOMATERIALS, V14, P817, DOI 10.1016/0142-9612(93)90003-K Shrivastava A., 2011, CHRON YOUNG SCI, V2, P21, DOI DOI 10.4103/2229-5186.79345 Shu PY, 2003, CLIN DIAGN LAB IMMUN, V10, P622, DOI 10.1128/CDLI.10.4.622-630.2003 Sombatmankhong K, 2007, POLYMER, V48, P1419, DOI 10.1016/j.polymer.2007.01.014 Tang C, 2014, J MEMBRANE SCI, V472, P251, DOI 10.1016/j.memsci.2014.08.037 Thomas L, 2010, AM J TROP MED HYG, V83, P696, DOI 10.4269/ajtmh.2010.10-0138, 10.1269/ajtmh.2010.10-0138 Wang C, 2011, LANGMUIR, V27, P12058, DOI 10.1021/la202267p Xu H, 2006, J CLIN MICROBIOL, V44, P2872, DOI 10.1128/JCM.00777-06 Yoon JY, 1996, J COLLOID INTERF SCI, V177, P613, DOI 10.1006/jcis.1996.0075 Zhang YZ, 2005, J MATER SCI-MATER M, V16, P933, DOI 10.1007/s10856-005-4428-x Hosseini, Samira Azari, Pedram Farahmand, Elham Gan, S. N. Rothan, Hussin A. Yusof, Rohana Koole, Leo H. Djordjevic, Ivan Ibrahim, Fatimah Engineering, Faculty /I-7935-2015 Engineering, Faculty /0000-0002-4848-7052 University of Malaya Flagship Grant FL001-14AET; High Impact Research Grant UM-MOHE UM; Ministry of Higher Education Malaysia C/625/1/HIR/MOHE/05; University of Malaya Research Grant UMRG: RP009E-13AET This research is supported by University of Malaya Flagship Grant (FL001-14AET), High Impact Research Grant UM-MOHE UM. C/625/1/HIR/MOHE/05 from the Ministry of Higher Education Malaysia and University of Malaya Research Grant (UMRG: RP009E-13AET). Authors would like to acknowledge Mr. Amir Hossein Hosseini for his technical contribution. 0 ELSEVIER ADVANCED TECHNOLOGY OXFORD BIOSENS BIOELECTRON |
| Uncontrolled Keywords: | Electrospun fibers, Polymer coating, Surface functional groups, Protein, immobilization, ELISA, NONSTRUCTURAL PROTEIN NS1, DENGUE VIRUS-INFECTIONS, SURFACE, FUNCTIONALIZATION, NANOFIBROUS MEMBRANES, IMMOBILIZATION, ANTIGEN, ELISA, MICROSPHERES, IMMUNOASSAY, RECOGNITION, |
| Subjects: | T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) |
| Divisions: | Faculty of Engineering |
| Depositing User: | Mr Jenal S |
| Date Deposited: | 09 Mar 2016 02:42 |
| Last Modified: | 01 Nov 2017 06:07 |
| URI: | http://eprints.um.edu.my/id/eprint/15688 |
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