Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods

Ali, M.E. and Razzak, M.A. and Abd Hamid, S.B. and Rahman, M.M. and Al Amin, M. and Abd Rashid, N.R. and Asing, - (2015) Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods. Food Chemistry, 177. pp. 214-224.

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Abstract

Food falsification has direct impact on public health, religious faith, fair-trades and wildlife. For the first time, here we described a multiplex polymerase chain reaction assay for the accurate identification of five meat species forbidden in Islamic foods in a single assay platform. Five pairs of species-specific primers were designed targeting mitochondrial ND5, ATPase 6, and cytochrome b genes to amplify 172, 163, 141, 129 and 108 bp DNA fragments from cat, dog, pig, monkey and rat meats, respectively. All PCR products were identified in gel-images and electrochromatograms obtained from Experion Bioanalyzer. Species-specificity checking against 15 important meat and fish and 5 plant species detected no cross-species amplification. Screening of target species in model and commercial meatballs reflected its application to detect target species in process foods. The assay was tested to detect 0.01-0.02 ng DNA under raw states and 1% suspected meats in meatball formulation. (C) 2015 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Islamic foods; Multiplex PCR; Halal and haram species; Fair-trade economy
Subjects: B Philosophy. Psychology. Religion > BP Islam. Bahaism. Theosophy, etc > Islam
Q Science > Q Science (General)
Depositing User: Mr Faizal 2
Date Deposited: 13 Apr 2015 02:43
Last Modified: 13 Apr 2015 02:43
URI: http://eprints.um.edu.my/id/eprint/13246

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