Characterization, antibacterial and in vitro compatibility of zinc-silver doped hydroxyapatite nanoparticles prepared through microwave synthesis

Iqbal, N. and Kadir, M.R.A. and Mahmood, N.H. and Salim, N. and Froemming, G.R.A. and Balaji, H.R. and Kamarul, Tunku (2014) Characterization, antibacterial and in vitro compatibility of zinc-silver doped hydroxyapatite nanoparticles prepared through microwave synthesis. Ceramics International, 40 (3). pp. 4507-4513. ISSN 0272-8842, DOI

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We investigated the possibility of enhancing hydroxyapatite (HA) bioactivity by co-substituting it with zinc and silver. Zn-Ag-HA nanoparticles were synthesized by using the microwave-assisted wet precipitation process, and their phase purity, elemental composition, morphology, and particle size were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). FTIR, XRD, and EDX results showed the characteristic peaks of the Zn-Ag-HA structure, while SEM results demonstrated that the nanoparticles were of spherical shape with a particle size of 70-102 nm. Antibacterial tests of the nanoparticles revealed their antibacterial activity against Staphylococcus aureus and Escherichia colt. By using simulated body fluid (SBF), an apatite layer formation was observed at 28 days. In vitro cell adhesion assay confirmed the cell attachment of normal human osteoblast (NHOst) cells to the disc surface. MTT (3(4, 5-dimethylthiazol-2-y1)-2, 5 diphenyhetrazolium bromide assay indicated that the cells were viable, and the cells proliferated faster on the disks than on the control surface due to the presence of metal ions. In conclusion, the novel Zn-Ag-HA nanoparticles were found to be compatible with in vitro experiments and having potential antibacterial properties. Therefore these nanoparticles could be a promising candidate for future biomedical applications. (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.

Item Type: Article
Additional Information: ISI Document Delivery No.: AA3TV Times Cited: 0 Cited Reference Count: 28 Iqbal, Nida Kadir, Mohammed Rafiq Abdul Mahmood, Nasrul Humaimi Salim, Norita Froemming, Gabriele R. A. Balaji, H. R. Kamarul, Tunku UTM 45027; Ministry of Higher Education (MOHE); RMC; University of Malaya HIR-MOHE The authors would like to thank Nik Ahmad Nazim Nik Malek (Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM) for his support in antibacterial testing. The authors would like to acknowledge the Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine UiTM Campus Sungai Buloh Selangor, Malaysia for providing cell culture facilities. The authors would like to acknowledge the financial support provided by UTM research Grant science funding (Vote: 45027), Ministry of Higher Education (MOHE) and RMC. More than one of the authors of this paper was supported under University of Malaya HIR-MOHE research grant. Elsevier sci ltd Oxford
Uncontrolled Keywords: Biomaterial; Bioactivity; Orthopedics; Antibacterial agent; Microwave; synthesis; substituted hydroxyapatite; temperature; nanopowders; bioactivity; infections; surface
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Haslinda Lahuddin
Date Deposited: 02 May 2014 01:30
Last Modified: 10 Oct 2018 08:58

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