Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates

Gumel, A.M. and Annuar, M.S.M. and Heidelberg, T. (2013) Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates. Journal of Chemical Technology and Biotechnology. pp. 1328-1335. DOI

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BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, temperature,enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1’-O-3-hydroxyacyl-sucrose) using Candida antarctica lipase B (EC studied. Using H2O2 as micro-initiator, enzyme-mediated synthesis yielded reaction rate, vapp of 0.076 x 10−5 mol L−1 s−1. The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non-functionalized material apart from showing better compostability. Increasing the reaction temperature (>50◦C), enzyme concentration (>15 g L−1) and reactant ratio (w/w) of sucrose:PHA (>2) did not increase further the rate or yield. The sucrose-functionalized mcl-PHAwas characterized with respect to the non-functionalized material. CONCLUSIONS: Novozym 435 can be used effectively to synthesize poly(1’-O-3-hydroxyacyl sucrose) in micro-aqueous medium bypassing the need for chemo-synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches

Item Type: Article
Additional Information: Correspondence to: SuffianMAnnuar, Institute of Biological Sciences, Faculty of Science, University ofMalaya, 50603 Kuala Lumpur, Malaysia. E-mail:
Uncontrolled Keywords: Biopolymer; Candida antarctica; Lipase; Polyhydroxyalkanoates; Sugar-ester; Sucrose.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Norhamizah Tamizi
Date Deposited: 22 Apr 2014 01:51
Last Modified: 22 Apr 2014 01:51

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