Evaluation of Toxoplasma gondii-recombinant dense granular protein (GRA2) for serodiagnosis by western blot

Ching, X.T. and Lau, Y.L. and Fong, M.Y. and Nissapatorn, V. (2013) Evaluation of Toxoplasma gondii-recombinant dense granular protein (GRA2) for serodiagnosis by western blot. Parasitology Research, 112 (3). pp. 1229-1236. ISSN 0932-0113,

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Toxoplasma gondii infects all warm-blooded animals including humans, causing serious public health problems and great economic loss in the food industry. Commonly used serological tests involve preparation of whole Toxoplasma lysate antigens from tachyzoites which are costly and hazardous. An alternative method for better antigen production involving the prokaryotic expression system was therefore used in this study. Recombinant dense granular protein, GRA2, was successfully cloned, expressed, and purified in Escherichia coli, BL21 (DE3) pLysS. The potential of this purified antigen for diagnosis of human infections was evaluated through western blot analysis against 100 human serum samples. Results showed that the rGRA2 protein has 100 and 61.5 sensitivity towards acute and chronic infection, respectively, in T. gondii-infected humans, indicating that this protein is useful in differentiating present and past infections. Therefore, it is suitable to be used as a sensitive and specific molecular marker for the serodiagnosis of Toxoplasma infection in both humans and animals.

Item Type: Article
Additional Information: ISI Document Delivery No.: 096ZH Times Cited: 0 Cited Reference Count: 30 Ching, Xiao Teng Lau, Yee Ling Fong, Mun Yik Nissapatorn, Veeranoot University of Malaya PS197-2009C, E000051-20001 Serum samples were kindly provided by the Diagnostic Laboratory (Para: SEAD), Department of Parasitology, University of Malaya and University Malaya Medical Centre. Special thanks to the UMCPR for carrying out the MALDI-TOF MS analysis. We would also like to thank Claudia Nisha Anthony for proofreading this article. This project was supported by the University of Malaya under the Postgraduate Research Grant (PS197-2009C) and High Impact Research Fund (E000051-20001). Springer New york
Uncontrolled Keywords: Immunoglobulin-G Parasitophorous Vacuole Pregnant-Women Antigens Antibodies Infection Sera Organelles Membrane Humans
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 30 Sep 2013 03:22
Last Modified: 13 Nov 2013 07:54
URI: http://eprints.um.edu.my/id/eprint/8415

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