Gender identification of mouse embryos using the polymerase chain reaction (PCR) technique

Abdullah, Ramli and Mohamed Said, M.S. and Nor Ashikin, M.N.K. and Froemming, G.A.R. and Mohamed, Z. and Nor Shahida, A.R. (2006) Gender identification of mouse embryos using the polymerase chain reaction (PCR) technique. Biomedical Research, 17 (3). pp. 193-195. ISSN 0970938X,

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A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female DNA gave a single band of 111 bp (Nds). Test on blood DNA showed a 98.0% PCR amplification efficiency whereas single blastomeres produced 93.0% PCR amplification efficiency. In conclusion, mouse embryos were successfully sexed within 6 hours by using single blastomeres obtained from 8-cell embryos.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Embryo biopsy, Embryo sexing, Gender identification, DNA, Amplicon, Animal cell, Animal experiment, Article, Blastomere, Chromosome analysis, Controlled study, Dna determination, Dna polymorphism, Embryo, Female, Gender identity, Gene amplification, Genetic analysis, Male, Mouse, Nonhuman, Polymerase chain reaction, Sequence analysis, Sex determination, SRY gene, X chromosome, Y chromosome
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 22 Oct 2013 04:06
Last Modified: 28 Apr 2021 07:41

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