Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture

Chong, T.E. and Teck, F.G. and Ming, W.S. and Rahman, N.A. and Khalid, N. and Karsani, S.A. and Othman, S. and Yusof, R. (2011) Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture. Journal of Medicinal Plants Research, 5 (16). pp. 3777-3780. ISSN 19960875,

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Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures.

Item Type: Article
Uncontrolled Keywords: Boesenbergia rotunda Callus Plant proteomics Temu kunci Two dimensional electrophoresis Boesenbergia rotunda extract enzyme plant extract protein proteome unclassified drug antibacterial activity antifungal activity antiinflammatory activity antineoplastic activity antioxidant activity antiviral activity article biosynthesis controlled study food in vitro study nonhuman proteomics reproducibility Southeast Asia suspension cell culture traditional medicine two dimensional gel electrophoresis Zingiberaceae
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 07 May 2013 02:03
Last Modified: 07 May 2013 02:03
URI: http://eprints.um.edu.my/id/eprint/5975

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