Advancements in detection approaches of severe acute respiratory syndrome coronavirus 2

Hossain, M. A. Motalib and Uddin, Syed Muhammad Kamal and Hashem, Abu and Al Mamun, Mohammad and Sagadevan, Suresh and Johan, Mohd Rafie (2022) Advancements in detection approaches of severe acute respiratory syndrome coronavirus 2. Malaysian Journal of Medical Sciences, 29 (6). 15 – 33. ISSN 1394-195X, DOI

Full text not available from this repository.


Diagnostic testing to identify individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in selecting appropriate treatments, saving people’s lives and preventing the global pandemic of COVID-19. By testing on a massive scale, some countries could successfully contain the disease spread. Since early viral detection may provide the best approach to curb the disease outbreak, the rapid and reliable detection of coronavirus (CoV) is therefore becoming increasingly important. Nucleic acid detection methods, especially real-time reverse transcription polymerase chain reaction (RT-PCR)-based assays are considered the gold standard for COVID-19 diagnostics. Some non-PCR-based molecular methods without thermocycler operation, such as isothermal nucleic acid amplification have been proved promising. Serologic immunoassays are also available. A variety of novel and improved methods based on biosensors, Clustered-Regularly Interspaced Short Palindromic Repeats (CRISPR) technology, lateral flow assay (LFA), microarray, aptamer etc. have also been developed. Several integrated, random-access, point-of-care (POC) molecular devices are rapidly emerging for quick and accurate detection of SARS-CoV-2 that can be used in the local hospitals and clinics. This review intends to summarize the currently available detection approaches of SARS-CoV-2, highlight gaps in existing diagnostic capacity, and propose potential solutions and thus may assist clinicians and researchers develop better technologies for rapid and authentic diagnosis of CoV infection. © 2022, Penerbit Universiti Sains Malaysia. All rights reserved.

Item Type: Article
Funders: Universiti Malaya [Grant no. RU001-2020, ST017-2020]
Uncontrolled Keywords: COVID-19; Detection approach; Nucleic acid; RT-qPCR; SARS-CoV-2
Subjects: Q Science > QD Chemistry
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 01 Dec 2023 07:18
Last Modified: 01 Dec 2023 07:18

Actions (login required)

View Item View Item