Molecular detection of pathogenic Leptospira spp. in urban rodents from wet markets in northeast Malaysia

Kamaruzaman, Intan Noor Aina and Mokhtar, Muhamad Aiman Mohd and Ting, Hong Wei and Yuan, Yong Kai and Shah, Azim Wafiy Gulam and Loong, Tan Wan and Shaharulnizim, Nurshahirah and Reduan, Mohd Farhan Hanif and Hamid, Fathin Faahimaah Abdul and Noralidin, Nur Amalina and Manaf, Nur Athirah Abdul and Zalati, Che Wan Salma Che Wan and Loong, Shih-Keng and Clegg, Simon and Abu-Bakar, Luqman (2022) Molecular detection of pathogenic Leptospira spp. in urban rodents from wet markets in northeast Malaysia. Journal of Advanced Veterinary and Animal Research, 9 (2). 275 -281. ISSN 2311-7710, DOI

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Objective: This short study describes the occurrence of pathogenic Leptospira spp. in two major wet markets in Kota Bharu, Kelantan, Malaysia. Materials and Methods: 30 rodents (20 rats and 10 shrews) were caught in 2 wet markets, and a postmortem was performed to extract both kidneys. Molecular diagnosis via polymerase chain reaction (PCR) was conducted to detect leptospiral DNA using universal and pathogenic Leptospira primers, respectively. Results: The results showed that 20/28 (72) rat samples were detected positive for Leptospira spp, and all shrews were negative. Further sequencing analysis identified L. interrogans and L. borgpetersenii as the most frequently Leptospirosis species from kidney samples. Conclusions: The presented study here sheds light on the presence of pathogenic leptospires harboring the rat population in both wet markets in Kelantan, which presents a great public health risk to wet market workers and visitors. © The authors. This is an Open Access article distributed under the terms ofthe Creative Commons Attribution 4.0 License (

Item Type: Article
Funders: Department of Medical Microbiology, Faculty of Medicine and Health Science Universiti Putra Malaysia
Uncontrolled Keywords: Leptospires; Malaysia; Molecular detection; Pcr; Rodents; Wet market
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 Oct 2023 05:20
Last Modified: 30 Oct 2023 05:20

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