Differential expression of collagen IV alpha 1 to alpha 6 chains in basement membranes of benign and malignant odontogenic tumors

Nagatsuka, H. and Siar, C.H. and Nakano, K. and Tsujigiwa, H. and Gunduz, M. and Choufuku, H. and Lee, Y.J. and Naito, I. and Sado, Y. and Nagai, N. (2002) Differential expression of collagen IV alpha 1 to alpha 6 chains in basement membranes of benign and malignant odontogenic tumors. Virchows Archiv, 441 (4). pp. 392-399. ISSN 0945-6317, DOI https://doi.org/10.1007/s00428-002-0658-1.


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Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of odontogenic neoplasms, immunohistochemistry using six anti-a(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular a(IV) chain profile in malignant odontogenic tumors as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic (n=3) than in ordinary (n=5) ameloblastomas. The adenomatoid odontogenic tumor (n=2) distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma (n=3) and ameloblastic fibro-odontosarcoma (n=1), but not the inductive hard tissues. Ameloblastic carcinoma (n=2) showed specific alpha1(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma (n=1) demonstrated a discontinuous alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IValpha chains occur during odontogenic neoplasms' progression.

Item Type: Article
Additional Information: ISI Document Delivery No.: 620MN Times Cited: 16 Cited Reference Count: 43 Cited References: BARSKY SH, 1983, LAB INVEST, V49, P140 Catusse C, 2000, J HISTOCHEM CYTOCHEM, V48, P663 Chelberg M.K., 1990, J CELL BIOL, V111, P262 Dehan P, 1997, AM J PATHOL, V151, P1097 Erickson AC, 2000, J HISTOCHEM CYTOCHEM, V48, P1291 Eversole LR, 1999, SEMIN DIAGN PATHOL, V16, P317 Fleischmajer R, 1997, J INVEST DERMATOL, V109, P527, DOI 10.1111/1523-1747.ep12336696 HEIKINHEIMO K, 1991, VIRCHOWS ARCH B, V61, P101 HEIKINHEIMO K, 1995, J DENT RES, V74, P1226 Hewitt RE, 1997, BRIT J CANCER, V75, P221, DOI 10.1038/bjc.1997.37 HUDSON BG, 1993, J BIOL CHEM, V268, P26033 Kalluri R, 2000, J BIOL CHEM, V275, P12719, DOI 10.1074/jbc.275.17.12719 KRAMER IRH, 2002, INT HISTOLOGICAL CLA Lohi J, 1997, INT J CANCER, V72, P43, DOI 10.1002/(SICI)1097-0215(19970703)72:1<43::AID-IJC6>3.0.CO;2-4 Melrose RJ, 1999, SEMIN DIAGN PATHOL, V16, P271 Murata M, 2000, J ORAL PATHOL MED, V29, P483, DOI 10.1034/j.1600-0714.2000.291002.x NADIMI H, 1986, J ORAL PATHOL MED, V15, P439, DOI 10.1111/j.1600-0714.1986.tb00654.x Nagai N, 2001, INT J DEV BIOL, V45, P827 NAGAI N, 1994, ORAL ONCOL, V30B, P191 Nakano K, 2001, J PATHOL, V194, P420, DOI 10.1002/path.928 Nakano S, 1999, LAB INVEST, V79, P281 NINOMIYA Y, 1995, J CELL BIOL, V130, P1219, DOI 10.1083/jcb.130.5.1219 PHILIPSEN HP, 1992, INT J ORAL MAX SURG, V21, P352, DOI 10.1016/S0901-5027(05)80761-1 ROHRBACH DH, 1993, MOL CELLULAR ASPECTS, P1 SADO Y, 1995, HISTOCHEM CELL BIOL, V104, P267, DOI 10.1007/BF01464322 Sado Y, 1998, J BIOCHEM-TOKYO, V123, P767 SAKU T, 1992, J ORAL PATHOL MED, V21, P113, DOI 10.1111/j.1600-0714.1992.tb00993.x Salo T, 1999, J ORAL PATHOL MED, V28, P337 SAUK JJ, 1985, J ORAL PATHOL MED, V14, P307, DOI 10.1111/j.1600-0714.1985.tb00498.x Seki T, 1998, HISTOCHEM CELL BIOL, V110, P359, DOI 10.1007/s004180050296 Slater LJ, 1999, SEMIN DIAGN PATHOL, V16, P325 Takahashi H, 2001, J ORAL PATHOL MED, V30, P237, DOI 10.1034/j.1600-0714.2001.300408.x Takata T, 2000, VIRCHOWS ARCH, V436, P319, DOI 10.1007/s004280050453 Takata T, 2000, J DENT RES, V79, P1608 Takata T, 2000, VIRCHOWS ARCH, V436, P324, DOI 10.1007/s004280050454 Tanaka K, 1997, HISTOCHEM J, V29, P563, DOI 10.1023/A:1026428010104 THESLEFF I, 1984, J ORAL PATHOL MED, V13, P85, DOI 10.1111/j.1600-0714.1984.tb01404.x THESLEFF I, 1995, INT J DEV BIOL, V39, P35 THESLEFF I, 1981, DEV BIOL, V81, P182, DOI 10.1016/0012-1606(81)90361-4 Thomas G, 2001, INT J ORAL MAX SURG, V30, P349, DOI 10.1054/ijom.2001.0069 Tomich CE, 1999, SEMIN DIAGN PATHOL, V16, P308 Yamamoto K, 1995, Eur J Cancer B Oral Oncol, V31B, P122, DOI 10.1016/0964-1955(94)00046-7 YURCHENCO PD, 1994, CURR OPIN CELL BIOL, V6, P674, DOI 10.1016/0955-0674(94)90093-0 Nagatsuka, H Siar, CH Nakano, K Tsujigiwa, H Gunduz, M Choufuku, H Lee, YJ Naito, I Sado, Y Nagai, N Springer-verlag New york
Uncontrolled Keywords: Type IV collagen basement membrane immunohistochemistry odontogenic tumors human fetal teeth immunohistochemical demonstration enamel proteins basal lamina carcinoma ameloblastomas localization breast tooth
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 09 Jan 2013 02:40
Last Modified: 09 Jan 2013 02:40
URI: http://eprints.um.edu.my/id/eprint/4293

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