MiR-107 inhibits the sprouting of intersegmental vessels of zebrafish embryos

Khor, Eng-Soon and Mohd Noor, Suzita and Wong, Pooi-Fong (2022) MiR-107 inhibits the sprouting of intersegmental vessels of zebrafish embryos. Protoplasma, 259 (3). pp. 691-702. ISSN 0033-183X, DOI https://doi.org/10.1007/s00709-021-01695-1.

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MicroRNAs (miRNAs) play important roles in various biological processes. Our previous study showed that inhibition of MTOR with rapamycin treatment suppressed human endothelial cell tube formation, concomitant with the down-regulation of miR-107. Similarly, inhibition of Ztor by rapamycin also suppressed vascular development in zebrafish embryos. To gain a better understanding of the role of miR-107 and MTOR in vascular development, the present study sought to validate its function by over-expressing miR-107 in zebrafish embryos via microinjection with mimic miR-107 duplexes. Alkaline phosphatase (ALP) staining was used to visualise blood vessels in the zebrafish embryo, and expressions of Pten, Ztor and Rap1 were investigated by immunoblotting. Over-expression of miR-107 in zebrafish embryos inhibited the sprouting of intersegmental vessels (ISVs) with concomitant down-regulation of phosphorylated Rps6 expression, which confirmed the inhibition of Ztor signalling. As expected, pten, which is the target of miR-107, was down-regulated. Interestingly, Rap1, a small GTPase protein that is involved in intersomitic vessels sprouting during angiogenesis, was also down-regulated when miR-107 was over-expressed. Overall, our findings suggest that miR-107 and Ztor-mediated suppression of vascular development in zebrafish embryo involves Rap1.

Item Type: Article
Funders: University of Malaya Research Grant, RG500-13HTM, PG306-2016A
Uncontrolled Keywords: Angiogenesis; miR-107; Zebrafish; Ztor; Pten; Rap1
Subjects: Q Science > QL Zoology
Divisions: Faculty of Medicine
Faculty of Medicine > Biomedical Science Department
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 19 Nov 2023 12:29
Last Modified: 19 Nov 2023 12:29
URI: http://eprints.um.edu.my/id/eprint/42439

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