Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: Advances, challenges, and opportunities

Hashem, Abu and Hossain, M. A. Motalib and Ab Rahman, Marlinda and Al Mamun, Mohammad and Sagadevan, Suresh and Shahnavaz, Zohreh and Simarani, Khanom and Johan, Mohd Rafie (2022) Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: Advances, challenges, and opportunities. Critical Reviews In Clinical Laboratory Sciences, 59 (3). pp. 156-177. ISSN 10408363, DOI

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Clinical diagnostic tests should be quick, reliable, simple to perform, and affordable for diagnosis and treatment of diseases. In this regard, owing to their novel properties, biosensors have attracted the attention of scientists as well as end-users. They are efficient, stable, and relatively cheap. Biosensors have broad applications in medical diagnosis, including point-of-care (POC) monitoring, forensics, and biomedical research. The electrochemical nucleic acid (NA) biosensor, the latest invention in this field, combines the sensitivity of electroanalytical methods with the inherent bioselectivity of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The NA biosensor exploits the affinity of single-stranded DNA/RNA for its complementary strand and is used to detect complementary sequences of NA based on hybridization. After the NA component in the sensor detects the analyte, a catalytic reaction or binding event that generates an electrical signal in the transducer ensues. Since 2000, much progress has been made in this field, but there are still numerous challenges. This critical review describes the advances, challenges, and prospects of NA-based electrochemical biosensors for clinical diagnosis. It includes the basic principles, classification, sensing enhancement strategies, and applications of biosensors as well as their advantages, limitations, and future prospects, and thus it should be useful to academics as well as industry in the improvement and application of EC NA biosensors.

Item Type: Article
Funders: Bangabandhu Science and Technology Fellowship Trust, Government of the People’s Republic of Bangladesh, Universiti Malaya [Grant No: RU001-2020, ST017-2020]
Uncontrolled Keywords: Biosensor; Electrochemical; Clinical diagnosis; Nucleic acid; Application
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science
Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 05 Oct 2023 08:18
Last Modified: 05 Oct 2023 08:18

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