Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219

Abd Rahman, Zuraida and Seman, Zulkifli Ahmad and Othman, Ayu Nazreena and Ab Ghaffar, Mohamad Bahagia and Ab Razak, Shahril and Yusof, Muhammad Fairuz Mohd and Nasir, Khairun Hisam and Ahmad, Khairulmazmi and Chow, Yeow Lit and How, Teo Chee and Md Saad, Norsharina and Subramaniam, Sreeramanan (2022) Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219. PLANT BIOTECHNOLOGY REPORTS, 16 (3). pp. 343-355. ISSN 1863-5466, DOI

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The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59-61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 degrees C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isolation protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 mu m and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets.

Item Type: Article
Funders: Ministry of Energy, Science, Technology, Environment and Climate Change Research & Development Fund (MESTECC) TF FP0214B055
Uncontrolled Keywords: Plantlet regeneration; Microspore culture; Uninucleate; Viability; Callus initiation
Subjects: S Agriculture > S Agriculture (General)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 16 Oct 2023 06:30
Last Modified: 24 Oct 2023 03:38

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