The therapeutic potential of gamma-Al2O3 nanoparticle containing 5-fluorouracil in the treatment of colorectal cancer

Fasihi, Khadije and Amerizadeh, Forouzan and Sabbaghzadeh, Reihaneh and Heydari, Maryam and Rahmani, Farzad and Mostafapour, Asma and Khazaei, Majid and Rasouli, Elisa and Hassanian, Seyed Mahdi and Ferns, Gordon A. and Rezayi, Majid and Avan, Amir (2022) The therapeutic potential of gamma-Al2O3 nanoparticle containing 5-fluorouracil in the treatment of colorectal cancer. Tissue & Cell, 76. ISSN 0040-8166, DOI

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5-Fluorouracil (5-FU) is being used in the treatment of several malignancies, but side effects are often reported and include: diarrhea, vomiting, nausea, poor appetite, watery eyes, and photophobia. We have developed and tested the cytotoxic activity of nanocrystalline powder of gamma-alumina (gamma-Al2O3) containing 5-FU in two-dimensional and three-dimensional (3D) CRC cell culture. gamma-Al2O3 was prepared using a facile sol-gel method. The physicochemical properties of nanoparticles were investigated by Fourier Transform Infrared (FTIR) analysis, Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray Analysis (EDXA). Moreover, the particle size was monitored by Transmission Electron Microscopy (TEM). We used MTT and a scratch assay to assess the antiproliferative and anti-migratory of this agent. The effect of gamma-Al2O3-5-FU on SOD, MDA, and total-thiols levels were evaluated. We assessed the expression of apoptotic markers in mRNA or proteins by RT-PCR and ELISA respectively. gamma-Al2O3-5-FU inhibited cell growth in two-dimensional (2D) and three-dimensional (3D) cell culture and increased apoptosis as detected by DAPI stainning via modulation of caspases, BAx, BCl2 and cyclinD1 . gamma-Al2O3-5-FU also reduced the migratory activity of CRC cells relative to untreated controls. gamma-Al2O3-5-FU increased the level of MDA, while reducing the level of SOD and total-thiols as well as inflamatory markers (e.g., TNF-s and IL-6). Our study demonstrated that gamma-Al2O3-5-FU inhibited cell growth and migration, indicating its potential value in the treatment of colorectal cancer.

Item Type: Article
Funders: National Institute for Medical Research Development [Grant No: 987541]
Uncontrolled Keywords: Gamma-Al2O3-5-FU; 5-FU; EDXA; FTIR; FESEM; Colon cancer
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 11 Oct 2023 07:22
Last Modified: 11 Oct 2023 07:22

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