Effect of carbonate content on the in vitro bioactivity of carbonated hydroxyapatite

Safarzadeh, Marjan and Chee, Chin Fei and Ramesh, Singh (2022) Effect of carbonate content on the in vitro bioactivity of carbonated hydroxyapatite. Ceramics International, 48 (13). pp. 18174-18179. ISSN 0272-8842, DOI https://doi.org/10.1016/j.ceramint.2022.03.076.

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The effect of carbonate content on the apatite-forming ability of carbonated hydroxyapatite (CHA) in simulated body fluid (SBF) has been investigated. Five different nanocrystalline B-type CHA with carbonate content ranged from 2.01 to 5.25 wt% were prepared, sintered, and assessed for their in vitro bioactivity in SBF solution for 7-weeks under static conditions at 36.5 degrees C. The formation of the apatite layer and the surface morphology of CHA were examined by using a scanning electron microscope (FESEM) at week 1, 3, and 7 of SBF immersion, respectively. The Ca/P molar ratio of the CHA was determined by X-ray fluorescence (XRF). In addition, the sample weight changes and the pH of the SBF solution were measured. The results show that the formation of apatite layer depends on the carbonate content of CHA. Increasing the carbonate content caused significant increases in the surface area of CHA and the rate of apatite formation. Weight loss was observed for all CHA samples during the first week of SBF immersion, and thereafter followed by weight regain weekly until week 7. The changes in the pH of SBF and the Ca/P molar ratio were proportional to the carbonate content of CHA. This study thus highlights the importance of determining carbonate content aspect that govern the bioactivity of CHA.

Item Type: Article
Funders: Universiti Malaya Research Grant (Grant No: RU001B-2021)
Uncontrolled Keywords: Bone substitute; In vitro bioactivity; Simulated body fluid; B-type CHA; Carbonate content
Subjects: T Technology > TJ Mechanical engineering and machinery
Divisions: Faculty of Engineering > Department of Mechanical Engineering
Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 16 Oct 2023 03:17
Last Modified: 16 Oct 2023 03:17
URI: http://eprints.um.edu.my/id/eprint/42163

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