UV-B-induced DNA damage and repair pathways in polar Pseudogymnoascus sp. from the Arctic and Antarctic regions and their effects on growth, pigmentation and conidiogenesis

Wong, Hao Jie and Mohamad-Fauzi, Nuradilla and Idid, Mohammed Rizman and Convey, Peter and Smykla, Jerzy and Alias, Siti Aisyah (2022) UV-B-induced DNA damage and repair pathways in polar Pseudogymnoascus sp. from the Arctic and Antarctic regions and their effects on growth, pigmentation and conidiogenesis. Environmental Microbiology, 24 (7). pp. 3164-3180. ISSN 1462-2912, DOI https://doi.org/10.1111/1462-2920.16073.

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Solar radiation regulates most biological activities on Earth. Prolonged exposure to solar UV radiation can cause deleterious effects by inducing two major types of DNA damage, namely, cyclobutane pyrimidine dimers (CPDs) and pyrimidine 6-4 pyrimidone photoproducts. These lesions may be repaired by the photoreactivation (Phr) and nucleotide excision repair (NER) pathways; however, the principal UV-induced DNA repair pathway is not known in the fungal genus Pseudogymnoascus. In this study, we demonstrated that an unweighted UV-B dosage of 1.6 kJ m(-2) d(-1) significantly reduced fungal growth rates (by between 22% and 35%) and inhibited conidia production in a 10 d exposure. The comparison of two DNA repair conditions, light or dark, which respectively induced photoreactivation (Phr) and NER, showed that the UV-B-induced CPDs were repaired significantly more rapidly in light than in dark conditions. The expression levels of two DNA repair genes, RAD2 and PHR1 (encoding a protein in NER and Phr respectively), demonstrated that NER rather than Phr was primarily activated for repairing UV-B-induced DNA damage in these Pseudogymnoascus strains. In contrast, Phr was inhibited after exposure to UV-B radiation, suggesting that PHR1 may have other functional roles. We present the first study to examine the capability of the Arctic and Antarctic Pseudogymnoascus sp. to perform photoreactivation and/or NER via RT-qPCR approaches, and also clarify the effects of light on UV-B-induced DNA damage repair in vivo by quantifying cyclobutene pyrimidine dimers and pyrimidine 6-4 pyrimidone photoproducts. Physiological response data, including relative growth rate, pigmentation and conidia production in these Pseudogymnoascus isolates exposed to UV-B radiation are also presented.

Item Type: Article
Funders: Malaysian Ministry of Higher Education (MOHE) through their Higher Centre of Excellence (HiCoE) funding programme (Grant No: IOES-2014G), Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia (Grant No: GA006-2014FL), Ministry of Higher Education - Polish Ministry of Science and Higher Education (Grant No: NN305376438), UK Research & Innovation (UKRI) Natural Environment Research Council (NERC)
Uncontrolled Keywords: Nucleotide excision-repair; Cyclobutane pyrimidine dimers; Beauveria-bassiana; Cellular-dna;pcr data; Radiation; Light; Gene; Mechanisms; Responses
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Institute of Advanced Studies
Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 27 Oct 2023 04:49
Last Modified: 27 Oct 2023 04:49
URI: http://eprints.um.edu.my/id/eprint/41676

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