Nanosensing colon cancer biomarker on zeolite-modified gap-fingered dielectrodes

Gao, Ming and Sun, Yuansong and Wang, Qi and Ma, Shuaiting and Guo, Xinwei and Zhou, Lingling and Chen, Yeng and Marimuthu, Kasi and Gopinath, Subash C. B. (2022) Nanosensing colon cancer biomarker on zeolite-modified gap-fingered dielectrodes. Biotechnology and Applied Biochemistry, 69 (5). pp. 1885-1892. ISSN 0885-4513, DOI

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Nanomaterial on the sensing area elevates the biomolecular immobilization by its right orientation with a proper alignment, and zeolite is one of the suitable materials. In this research, the zeolite nanoparticles were synthesized using rice hush ash as the basic source and the prepared zeolite by the addition of sodium silicate was utilized to attach antibody as a probe on a gap-fingered dielectrode surface to identify the colon cancer biomarker, ``colon cancer-secreted protein-2'' (CCSP-2). Field Emission Scanning Electron Microscopy and Field Emission Transmission Electron Microscopy images confirmed the size of the nanoparticle to be similar to 15 nm and the occurrence of silica and alumina. Zeolite was modified on the electrode surface through the amine linker, and then anti-CCSP-2 was attached by an aldehyde linker. On this surface, CCSP-2 was detected and attained the detection limit to be 3 nM on the linear regression curve with 3-5 nM of CCSP-2. Estimated by the determination coefficient of y = 2.3952x - 4.4869 and R-2 = 9041 with 3 delta (n = 3). In addition, control proteins did not produce the notable current response representing the specific sensing of CCSP-2. This research is suitable to identify CCSP-2 at a lower level in the bloodstream under the physiological condition of a colon cancer patient.

Item Type: Article
Uncontrolled Keywords: Cancer stages; Colon cancer secreted protein-2; Dielectrode; Silica-aluminate
Subjects: R Medicine
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Dentistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 Aug 2023 03:51
Last Modified: 30 Aug 2023 03:51

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