First record of the diatom Nitzschia navis-varingica (Bacillariophyceae) producing amnesic shellfish poisoning-toxins from Papua New Guinea

Puilingi, Clyde Gorapava and Tan, Suh Nih and Maeno, Yukari and Leaw, Chui Pin and Lim, Po Teen and Yotsu-Yamashita, Mari and Terada, Ryuta and Kotaki, Yuichi (2022) First record of the diatom Nitzschia navis-varingica (Bacillariophyceae) producing amnesic shellfish poisoning-toxins from Papua New Guinea. Toxicon, 216. pp. 65-72. ISSN 0041-0101, DOI

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To determine the species distribution of an amnesic shellfish poisoning (ASP) toxins-producing diatom Nitzschia navis-varingica outside its current restricted geographical distribution range in Asian coastal waters, samples were collected from two sites of Bootless Bay, located on southwest coast of Papua New Guinea near Port Moresby. A total of twenty-one strains of N. navis-varingica were isolated and the clonal cultures established. The species identity was confirmed by molecular characterization based on the ribosomal DNA markers. The LSU rDNA phylogenetic inference revealed a monophyletic clade of all strains, clustered with N. navis-varingica with high bootstrap supports. ASP toxin production in the strains was investigated by HPLC with fluorescence detection and subsequently confirmed for the representative isolates by LC-MS/MS with multiple reaction monitoring (MRM) mode. All eleven strains from site A showed presence of domoic acid (DA) and isodomoic acid (IB); the toxin quota ranged from 0.70 to 4.63 pg cell-1 (average 2.75 +/- 1.26 pg cell-1, n = 11), with the composition of DA and IB of 21 DA: 79 IB. While for strains from site B, four out of ten strains showed presence of DA and IB, with the toxin quota ranged from 1.40 to 3.84 (average 2.57 +/- 1.17 pg cell-1, n = 4); the composition was 52 DA: 48 IB. The strains examined in this study were divided into toxic and probably non-toxic groups in ITS2 phylogeny. This represents the first record of domoic acid-producing Nitzschia navis-varingica from Papua New Guinea.

Item Type: Article
Funders: Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science [16H05797], Malaysian Ministry of Higher Education HiCOE [IOES-2014C], Long-term Research Grant Scheme [LRGS/1/2020/UMT/01/1/3]
Uncontrolled Keywords: Nitzschia navis-varingica; Domoic acid; Isodomoic acid B; ITS2; LSU rDNA; Phylogeny
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 28 Aug 2023 04:09
Last Modified: 28 Aug 2023 04:09

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