Genotoxicity of euphorbia hirta: an allium cepa assay

Ping, K.Y. and Darah, I. and Yusuf, U.K. and Yeng, C. and Sasidharan, S. (2012) Genotoxicity of euphorbia hirta: an allium cepa assay. Molecules, 17 (7). pp. 7782-7791. ISSN 1420-3049, DOI


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The potential genotoxic effects of methanolic extracts of Euphorbia hirta which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using Allium cepa assay. The extracts of 125, 250, 500 and 1,000 mu g/mL were tested on root meristems of A. cepa. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of E. hirta extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of E. hirta exerted significant genotoxic and mitodepressive effects at 1,000 mu g/mL.

Item Type: Article
Additional Information: ISI Document Delivery No.: 978OI Times Cited: 0 Cited Reference Count: 19 Cited References: Akinboro A, 2007, J ETHNOPHARMACOL, V112, P470, DOI 10.1016/j.jep.2007.04.014 Carilta R, 2008, CHEMOSPHERE, V72, P722, DOI 10.1016/j.chemosphere.2008.03.056 Christopher H.B., 1988, CYTOLOGIA, V54, P203 FISKESJO G, 1985, HEREDITAS, V102, P99, DOI 10.1111/j.1601-5223.1985.tb00471.x GRANT WF, 1982, MUTAT RES, V99, P273, DOI 10.1016/0165-1110(82)90046-X KABARITY A, 1974, BIOL PLANTARUM, V16, P275, DOI 10.1007/BF02921238 KLASTERSKA I, 1976, HEREDITAS, V83, P153 LANHERS MC, 1991, PLANTA MED, V57, P225, DOI 10.1055/s-2006-960079 Leme DM, 2009, MUTAT RES-REV MUTAT, V682, P71, DOI 10.1016/j.mrrev.2009.06.002 MCGILL M, 1974, CHROMOSOMA, V47, P157, DOI 10.1007/BF00331803 MERCYKUTTY VC, 1980, CYTOLOGIA, V45, P769 Odeigah PGC, 1997, HEREDITAS, V126, P161, DOI 10.1111/j.1601-5223.1997.00161.x Ogbolie J.N., 2007, AFR J BIOTECHNOL, V6, P1544 Rank J, 1997, MUTAT RES-GEN TOX EN, V390, P121, DOI 10.1016/S0165-1218(97)00008-6 Sandeep B.P., 2009, J PHARM RES HLTH CAR, V1, P113 Sharma A.K., 1980, CHROMOSOME TECHNIQUE, P474 SHEHAB AS, 1983, CYTOLOGIA, V48, P343 Soetan KO, 2009, J MED PLANTS RES, V3, P324 Yi HL, 2003, MUTAT RES-GEN TOX EN, V537, P109, DOI 10.1016/S1383-5718(03)00054-8 Ping, Kwan Yuet Darah, Ibrahim Yusuf, Umi Kalsom Yeng, Chen Sasidharan, Sreenivasan Ministry of Higher Education of Malaysian Government Kwan Yuet Ping is supported by the MyPhD fellowship from Ministry of Higher Education of Malaysian Government. Mdpi ag Basel
Uncontrolled Keywords: genotoxicity Allium cepa ethylmethane sulfonate mitotic index chromosome aberrations medicinal-plants chromosome stickiness root-tips aberrations mitosis water
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 10 Dec 2012 01:18
Last Modified: 10 Dec 2012 01:18

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