Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients

Othman, N. and Mohamed, Z. and Verweij, J.J. and Huat, L.B. and Olivos-Garcia, A. and Yeng, C. and Noordin, R. (2010) Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients. Foodborne Pathogens and Disease, 7 (6). pp. 637-641. ISSN 1535-3141, DOI


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Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7) pus samples, when compared with 14 of 30 (46.7) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess.

Item Type: Article
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Uncontrolled Keywords: Diagnosis amebiasis pcr antigen samples assay ala
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Ms Nursyafiqah Abd Malek
Date Deposited: 05 Dec 2012 03:39
Last Modified: 05 Dec 2012 03:39

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