Abiotic and biotic factors controlling sexual reproduction in populations of Pseudo-nitzschia pungens (Bacillariophyceae)

Kim, Jin Ho and Ajani, Penelope A. and Murray, Shauna A. and Kang, Su-Min and Kim, Sae-Hee and Lim, Hong Chang and Teng, Sing Tung and Lim, Po Teen and Park, Bum Soo (2023) Abiotic and biotic factors controlling sexual reproduction in populations of Pseudo-nitzschia pungens (Bacillariophyceae). Harmful Algae, 123. ISSN 1568-9883, DOI https://doi.org/10.1016/j.hal.2023.102392.

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Pseudo-nitzschia pungens is a widely distributed marine pennate diatom. Hybrid zones, regions in which two different genotypes may interbreed, are important areas for speciation and ecology, and have been reported across the globe for this species. However, sexual reproduction between differing clades in the natural envi-ronment is yet to be observed and is difficult to predict. Here we carried out experiments using two mono-clonal cultures of P. pungens from different genotypes to measure the frequency and timing of sexual reproduction across varying biotic (growth phases and cell activity potential) and abiotic conditions (nutrients, light, turbu-lence). We found the mating rates and number of zygotes gradually decreased from exponential to late stationary growth phases. The maximum zygote abundance observed was 1,390 cells mL-1 and the maximum mating rate was 7.1%, both which occurred during the exponential growth phase. Conversely, only 9 cells mL-1 and a maximum mating rate of 0.1% was observed during the late stationary phase. We also found the higher the relative potential cell activity (rPCA) in parent cells, as determined by the concentration of chlorophyll a per cell and the ratio of colony formation during parent cultivations, revealed higher mating rates. Furthermore, sexual events were reduced under nutrient enrichment conditions, and mating pairs and zygotes were not formed under aphotic (dark) or shaking culture conditions (150 rpm). In order to understand the sexual reproduction of Pseudo-nitzschia in the natural environment, our results highlight that it is most likely the combination of both biotic (growth phase, Chl. a content) and abiotic factors (nutrients, light, turbulence) that will determine the successful union of intraspecific populations of P. pungens in any given region.

Item Type: Article
Funders: Ministry of Education (MOE), Republic of Korea National Research Foundation of Korea (2019R1A6A1A10072987), National Research Foundation of Korea Ministry of Science, ICT & Future Planning, Republic of Korea Ministry of Science & ICT (MSIT), Republic of Korea (2022R1C1C1003582), Research Fund of Hanyang University (HY-202200000003109)
Uncontrolled Keywords: Pseudo-nitzschia pungens; Sexual reproduction; Mating rate; Potential cell activity
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Institute of Ocean and Earth Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 07 Jul 2023 07:54
Last Modified: 07 Jul 2023 07:54
URI: http://eprints.um.edu.my/id/eprint/38625

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