Chitinasiproducens palmae gen. nov., sp. nov., a new member of the family Burkholderiaceae isolated from leaf tissues of oil palm (Elaeis guineensis Jacq.)

Madhaiyan, Munusamy and See-Too, Wah-Seng and Ee, Robson and Saravanan, Venkatakrishnan Sivaraj and Wirth, Joseph S. and Alex, Tan Hian Hwee and Lin, Cai and Kim, Soo-Jin and Weon, Hang-Yeon and Kwon, Soon-Wo and Whitman, William B. and Ji, Lianghui (2020) Chitinasiproducens palmae gen. nov., sp. nov., a new member of the family Burkholderiaceae isolated from leaf tissues of oil palm (Elaeis guineensis Jacq.). International Journal of Systematic and Evolutionary Microbiology, 70 (4). pp. 2640-2647. ISSN 1466-5026, DOI

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A Gram-stain-negative, aerobic, rod-shaped, leaf-associated bacterium, designated JS23T, was isolated from surface-sterilized leaf tissue of an oil palm grown in Singapore and was investigated by polyphasic taxonomy. Phylogenetic analyses based on 16S rRNA gene sequences and 180 conserved genes in the genome of several members of Burkholderiaceae revealed that strain JS23(T) formed a distinct evolutionary lineage independent of other taxa within the family Burkholderiaceae. The predominant ubiquinone was Q-8. The primary polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified aminophospholipid. The major fatty acids were C-1(6:)0, summed feature 3 (C-16(:1)omega 7c/C-16:1 omega 6c) and summed feature 8 (C-1(8:)1 omega 7c/C-18:1 omega 6c). The size of the genome is 5.36 Mbp with a DNA G+C content of 66.2 mol%. Genomic relatedness measurements such as average nucleotide identity, genome-to-genome distance and digital DNA-DNA hybridization clearly distinguished strain JS23(T) from the closely related genera Burkholderia. Caballeronia, Mycetohabitans, Mycoayidus, Pandoraea, Paraburkholderia, Robbsia and Trinickia. Furthermore, average amino acid identity values and the percentages of conserved proteins, 56.0-68.4 and 28.2-45.5, respectively, were well below threshold values for genus delineation and supported the assignment of JS23(T) to a novel genus. On the basis of the phylogenetic, biochemical, chemotaxonomic and phylogenomic evidence, strain JS23(T) is proposed to represent a novel species of a new genus within the family Burkholderiaceae, for which the name Chitinasiproducens palmae gen. nov., sp. nov., is proposed with the type strain of JS23(T) (=DSM 27307(T)=KACC 17592(T)).

Item Type: Article
Funders: Temasek Foundation, Singapore Economic Development Board
Uncontrolled Keywords: Proteobacteria; chitinase; Burkholderia; Paraburkholderia; Mycoavidus
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 31 May 2023 06:30
Last Modified: 31 May 2023 06:30

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