Bioactive proteins in Channa striata promote wound healing through angiogenesis and cell proliferation

Kwan, Soon Hong and Aziz, Nur Hidayah Kaz Abdul and Ismail, Mohd Nazri (2020) Bioactive proteins in Channa striata promote wound healing through angiogenesis and cell proliferation. Protein and Peptide Letters, 27 (1). pp. 48-59. ISSN 0929-8665, DOI

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Background: Channa striata are speculated to contain bioactive proteins with the ability to enhancing wound healing. It is commonly consumed after surgery for a faster recovery of the wound. Objective: To identify the bioactive proteins and evaluate their ability in cell proliferation and angiogenesis promotion. Material and Methods: Freeze-Dried Water Extracts (FDWE) and Spray-Dried Water Extracts (SDWE) of C. striata were tested with MTT assay using EA.hy926 endothelial cell line and ex-vivo aortic ring assay. Later the proteins were fractionated and analysed using an LC-QTOF mass spectrometer. The data generated were matched with human gene database for protein similarity and pathway identification. Results: Both samples have shown positive cell proliferation and pro-angiogenic activity. Four essential proteins/genes were identified, which are collagen type XI, actin 1, myosin light chain and myosin heavy chain. The pathways discovered that related to these proteins are integrin pathway, Slit-Robo signalling pathway and immune response C-C Chemokine Receptor-3 signalling pathway in eosinophils, which contribute towards wound healing mechanism. Conclusions: The results presented have demonstrated that C. striata FDWE and SDWE protein fractions contain bioactive proteins that are highly similar to human proteins and thus could be involved in the wound healing process via specific biological pathways.

Item Type: Article
Funders: Universiti Sains Malaysia through the USM Bridging Grant 2017 (304.CABR.6316042), Fundamental Research Grant Scheme, Ministry of Higher Education Malaysia (203.CABR. 6711674)
Uncontrolled Keywords: Angiogenesis; Bioactive; Bioinformatics; Cell proliferation; Channa striata; Protein; Wound healing
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 01 Jun 2023 02:47
Last Modified: 01 Jun 2023 02:47

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