Elevated plasma and synovial fluid interleukin-8 and interleukin-18 may be associated with the pathogenesis of knee osteoarthritis

Koh, S. M. and Chan, C. K. and Teo, S. H. and Singh, S. and Merican, A. and Ng, W. M. and Abbas, A. and Kamarul, T. (2020) Elevated plasma and synovial fluid interleukin-8 and interleukin-18 may be associated with the pathogenesis of knee osteoarthritis. The Knee, 27 (1). pp. 26-35. ISSN 0968-0160, DOI https://doi.org/10.1016/j.knee.2019.10.028.

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Purpose: Osteoarthritis (OA) of the knee is a multifactorial degenerative disease typically defined as the `wear and tear' of articular joint cartilage. However, recent studies suggest that OA is a disease arising from chronic low-grade inflammation. We conducted a study to investigate the relationship between chronic inflammatory mediators present in both the systemic peripheral blood system and localised inflammation in synovial fluid (SF) of OA and non-OA knees; and subsequently made direct comparative analyses to understand the mechanisms that may underpin the processes involved in OA. Methods: 20-Plex proteins were quantified using Human Magnetic Luminex (R) assay (R&D Systems, USA) from plasma and SF of OA (n = 14) and non-OA (n = 14) patients. Ingenuity Pathway Analysis (IPA) software was used to predict the relationship and possible interaction of molecules pertaining to OA. Results: There were significant differences in plasma level for matrix metalloproteinase (MMP)-3, interleukin (IL)-27, IL-8, IL-4, tumour necrosis factor-alpha, MMP-1, IL-15, IL-21, IL-10, and IL-1 beta between the groups, as well as significant differences in SF level for IL-15, IL-8, vascular endothelial growth factor (VEGF), MMP-1, and IL-18. Our predictive OA model demonstrated that toll-like receptor (TLR) 2, macrophage migration inhibitory factor (MIF), TLR4 and IL-1 were the main regulators of IL-1B, IL-4, IL-8, IL-10, IL-15, IL-21, IL-27, MMP-1 and MMP-3 in the plasma system; whilst IL-1B, TLR4, IL-1, and basigin (BSG) were the regulators of IL-4, IL-8, IL-10, IL-15, IL-18, IL-21, IL-27, MMP-1, and MMP-3 in the SF system. Conclusion: The elevated plasma IL-8 and SF IL-18 may be associated with the pathogenesis of OA via the activation of MMP-3. (C) 2019 Published by Elsevier B.V.

Item Type: Article
Funders: Universiti Malaya (UM.c/625/1/HIR/MOHE/CHAN/03), Universiti Malaya (UMRG-RP005A-13HTM), Universiti Malaya (A000003-50001)
Uncontrolled Keywords: Inflammation; Osteoarthritis; Blood; Synovial fluid; Pathway
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms Zaharah Ramly
Date Deposited: 01 Jun 2023 04:30
Last Modified: 01 Jun 2023 04:30
URI: http://eprints.um.edu.my/id/eprint/37068

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