Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi

Muh, Fauzi and Kim, Namhyeok and Nyunt, Myat Htut and Firdaus, Egy Rahman and Han, Jin-Hee and Hoque, Mohammad Rafiul and Lee, Seong-Kyun and Park, Ji-Hoon and Moon, Robert W. and Lau, Yee Ling and Kaneko, Osamu and Han, Eun-Taek (2020) Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi. PLoS Neglected Tropical Diseases, 14 (6). ISSN 1935-2727, DOI

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Malaria is caused by multiple different species of protozoan parasites, and interventions in the pre-elimination phase can lead to drastic changes in the proportion of each species causing malaria. In endemic areas, cross-reactivity may play an important role in the protection and blocking transmission. Thus, successful control of one species could lead to an increase in other parasite species. A few studies have reported cross-reactivity producing cross-immunity, but the extent of cross-reactive, particularly between closely related species, is poorly understood.P.vivaxandP.knowlesiare particularly closely related species causing malaria infections in SE Asia, and whilstP.vivaxcases are in decline, zoonoticP.knowlesiinfections are rising in some areas. In this study, the cross-species reactivity and growth inhibition activity ofP.vivaxblood-stage antigen-specific antibodies againstP.knowlesiparasites were investigated. Bioinformatics analysis, immunofluorescence assay, western blotting, protein microarray, and growth inhibition assay were performed to investigate the cross-reactivity.P.vivaxblood-stage antigen-specific antibodies recognized the molecules located on the surface or released from apical organelles ofP.knowlesimerozoites. RecombinantP.vivaxandP.knowlesiproteins were also recognized byP.knowlesi-andP.vivax-infected patient antibodies, respectively. Immunoglobulin G againstP.vivaxantigens from both immune animals and human malaria patients inhibited the erythrocyte invasion byP.knowlesi. This study demonstrates that there is extensive cross-reactivity between antibodies againstP.vivaxtoP.knowlesiin the blood stage, and these antibodies can potently inhibit in vitro invasion, highlighting the potential cross-protective immunity in endemic areas. Author summary In recent years, malaria initiatives have increasingly shifted focus from achieving malaria control to achieving malaria elimination. However, the interventions used are leading to drastic changes in the proportions of differentPlasmodiumspecies causing clinical infection, particularly within Southeast Asia. Little is known about how these different parasite species interact/compete in nature or whether exposure to one species could cause some level of protection against another. We examined cross-reactive antibody responses to key parasite proteins with roles in red blood cell invasion and identified novel cross-species reactivity among the closest of malaria affecting the human population (P.vivaxandP.knowlesi). This comprehensive analysis provides evidence that cross-reactive immunity could play an important role in areas where species distributions are perturbed by malaria control measures, and future efforts to identify the specific cross-reactive epitopes involved would be invaluable both to our understanding of malaria immunity and vaccine development.

Item Type: Article
Uncontrolled Keywords: Animals; Antibodies, Protozoan; Antigens, Protozoan; Cross Reactions; Humans; Malaria;
Subjects: R Medicine > R Medicine (General)
R Medicine > RX Homeopathy > Diseases, treatment, etc
Divisions: Faculty of Medicine
Faculty of Medicine > Parasitology Deparment
Depositing User: Ms Zaharah Ramly
Date Deposited: 31 Dec 2023 02:38
Last Modified: 31 Dec 2023 02:38

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