PEGylated DPPC/Anti-SNAP25 antibody targeted liposomes from langmuir monolayer study to formulations

Gew, L.T. and Misran, Misni (2021) PEGylated DPPC/Anti-SNAP25 antibody targeted liposomes from langmuir monolayer study to formulations. Current Chemical Biology, 15 (3). pp. 249-261. ISSN 22127968, DOI

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Background: Molecule compatibility is an important factor to be considered before pre-paring antibody-targeted liposomes, stealth-liposomes, and stealth antibody-targeted liposomes. Objective: To determine the intermolecular interaction of 1,2-dioleoyl-sn-glycero-3-phosphoethano-lamide-N-methoxy(polyethyleneglycol)-2000 (ammonium salt), DOPE PEG2000 and Anti-S-NAP25 (AS25) in 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) monolayer, and their li-posomes. Methods: In this study, DPPC was used to create a monolayer mimicking the half membrane of li-posomes to investigate its interactions with a polyclonal antibody, AS25, and DOPE PEG2000, which are based on Langmuir-Blodgett (LB) techniques. The surface morphology of DPPC-AS25 and DPPC-DOPE PEG2000-AS25 bilayers were also imaged and analyzed by using atomic force microscopy (AFM) to support the LB findings. The LB findings were then utilized as a reference to prepare DPPC liposomes in this work. Results: The best mole ratio of DPPC-DOPE PEG2000, determined to be 50 to 1, was used to study the interaction with the polyclonal antibody AS25. The free energy of mixing (∆Gmix ) of DP-PC-DOPE PEG2000-AS25 was more negative than DPPC-AS25 in the entire investigated ranges, indicating that the ternary mixture of DPPC-DOPE PEG2000-AS25 was more compatible than the binary mixture of DPPC-AS25. The presence of DOPE PEG2000 in DPPC-AS25 increased the fluidity of the membrane, which resulted in a greater interaction of AS25 with DPPC. Conclusion: The constant values of particle size and zeta potential measurements of DPPC-DOPE PEG2000-AS25 liposomes showed agreement with the LB findings, indicating that LB is a good technique to predict precise liposomal formulations. © 2021.

Item Type: Article
Uncontrolled Keywords: 1,2 dipalmitoyl sn glycero 3 phosphorylcholine; alcohol; antibody; drug; liposome; macrogol 2000; synaptosomal associated protein 25 antibody; unclassified drug, Article; atomic force microscopy; drug formulation; isotherm; Langmuir Blodgett film; nonhuman; particle size; transmission electron microscopy; zeta potential
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Faculty of Science
Faculty of Science > Department of Chemistry
Depositing User: Ms Zaharah Ramly
Date Deposited: 28 Nov 2023 04:07
Last Modified: 29 Nov 2023 04:21

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